Fujian Provincial University Engineering Research Center of Green Materials and Chemical Engineering, College of Materials and Chemical Engineering, Minjiang University, Fuzhou, 350108, People's Republic of China.
Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou, 350108, People's Republic of China.
Mikrochim Acta. 2024 Sep 27;191(10):630. doi: 10.1007/s00604-024-06721-6.
A ratiometric self-powered photoelectrochemical sensor based on laser direct writing technology was constructed to address the problem that the conventional single-signal detection mode was susceptible to the influence of instrumentation and environmental factors, which interfered with the detection results. Laser-induced CdS/TiO/Graphene was prepared as dual photoanodes (PA1 and PA2), which were controlled by multiplexed switches to form a photocatalytic fuel cell with Pt cathode. By modifying the aptamer of aflatoxin B1 (AFB1) on the photoanode surface, the target was specifically captured to the electrode surface to form a biological complex, which increased the steric hindrance and affected the electron transfer, thus reducing the output signal of the sensor. Targets with different concentrations were incubated on the surface of PA1, and targets with fixed concentrations were incubated on the surface of PA2. Under the control of the multiplex switch, the output signals of the two photoanodes were recorded, and the ratio of these two signals was used as the basis for the quantitative detection of AFB1. The sensor output was linearly increasing with the logarithm of AFB1 concentration from 1.0 to 150 ng mL and the detection limit was 0.0974 ng mL. Additionally, this method had good stability, fast response, and good selectivity to real samples, providing an effective method for food safety monitoring.
基于激光直写技术构建了一种比率型自供电光电化学传感器，以解决传统单信号检测模式易受仪器和环境因素影响的问题，这些因素会干扰检测结果。制备了激光诱导的 CdS/TiO/Graphene 作为双光阳极（PA1 和 PA2），通过多路复用开关进行控制，形成具有 Pt 阴极的光催化燃料电池。通过在光阳极表面修饰黄曲霉毒素 B1（AFB1）的适体，目标物被特异性捕获到电极表面形成生物复合物，增加了空间位阻并影响了电子转移，从而降低了传感器的输出信号。将不同浓度的目标物孵育在 PA1 表面，将固定浓度的目标物孵育在 PA2 表面。在多路复用开关的控制下，记录两个光阳极的输出信号，并将这两个信号的比值用作 AFB1 定量检测的基础。传感器的输出与 AFB1 浓度的对数从 1.0 到 150 ng mL 呈线性增加，检测限为 0.0974 ng mL。此外，该方法对实际样品具有良好的稳定性、快速响应和良好的选择性，为食品安全监测提供了一种有效方法。
