[Histomorphological changes in the follicle system in the ovaries of ewes after irradiation].

Histological changes in primary follicles of ewes after the five-day protracted exposure to gamma rays were studied by qualitative and micrometric methods. The experiment was carried out in the anoestrous period with 21 ewes of the Slovak Merino breed, divided into three groups. The first control group (five ewes) was not irradiated. The second and third groups (each included eight ewes) were irradiated by gamma rays 60Co during the period of five days by the total dose of 4.8 Gy. All ewes included the control ones, were given Ampicillin Spofa 250 mg per head/day during the period of ten days after irradiation. The third group was administered, apart from this treatment, a mixture of vitamins Roboran H at the dose of 10 g per head/day. The animals were slaughtered on the fifth day of irradiation and on the tenth day after the end of irradiation. The ovaries, processed by a routine histological method, were cut in 7 micrometers slices in the series of 70 micrometers and stained with hematoxylin-eosine. By a qualitative histomorphological analysis of the oocytes of primary follicles chromatin aggregation, pycnosis of nuclei, pronounced acidophilia of oocyte cytoplasm, their shrinking and disintegration were determined in intact primary follicles, mitotic division of follicular cells stopped and the proportion of follicular cells with pycnotic nuclei increased after irradiation. The results show that the five-day protracted exposure to gamma rays at the total dose of 4.8 Gy causes pronounced degenerative changes in the anoestrous period.(ABSTRACT TRUNCATED AT 250 WORDS)