Staníková A, Arendarcik J, Tokos M, Balun J, Chlebovský O
Vet Med (Praha). 1983 Sep;28(9):529-40.
Caryometric analysis was used for the study of changes in the cell nucleus volume of the neurons of nucleus paraventricularis, nucleus arcuatus, and ependyma of the third cerebral ventricle of sheep after the administration of Gn-RH, followed by exposure to X-rays. The test animals were 12 ewes in physiological anoestrus and two rams. The trials were conducted in spring. The first group of four sheep and two rams were left as controls; in the ewes of the second group the hypothalamo-hypophysial region was irradiated by exposure to 516.5 mC/kg (200 R); in the four ewes of the third group, ovaries were directly irradiated at laparotomy by exposure to 64.4 mC/kg (250 R). The ewes of the second and third group were treated with an i. m. administration of 400 micrograms Gn-RH per head before irradiation. The excisions were collected and processed the tenth day from irradiation. The studied material was fixed by injection of 10% formalin and finished by another dose of 10% formalin after the excision of the brains. The paraffin slices were stained with haematoxylin-eosine. The caryometric analysis was performed at 3000-fold magnification, 200 cells being measured in each sample. Changes in neurosecretory cells were described in the regions of nucleus paraventricularis, nucleus arcuatus and in the ependyma of the third cerebral ventricle. The results of the caryometric analysis of nucleus paraventricularis and nucleus arcuatus suggest that the administration of Gn-RH and irradiation of the hypothalamo-hypophysial region, and direct irradiation of ovaries, stimulate the studied cerebral structures. The changes observed in the ependyma of the third cerebral ventricle after the administration of Gn-RH and subsequent irradiation of the hypothalamo-hypophysial region are insignificant; it is only after the direct irradiation of ovaries that these cells are inhibited by an indirect effect through the feedback mechanism.
采用细胞核测量分析法，研究给绵羊注射促性腺激素释放激素（Gn-RH）后再进行X射线照射，其室旁核、弓状核以及第三脑室室管膜神经元细胞核体积的变化。实验动物为12只处于生理性乏情期的母羊和2只公羊。实验在春季进行。第一组包括4只绵羊和2只公羊作为对照组；第二组母羊的下丘脑-垂体区域接受516.5毫库仑/千克（200伦琴）的照射；第三组的4只母羊在剖腹手术时对卵巢直接进行64.4毫库仑/千克（250伦琴）的照射。第二组和第三组的母羊在照射前每只肌肉注射400微克Gn-RH。在照射后第10天采集并处理标本。所研究的材料通过注射10%的福尔马林固定，大脑切除后再用另一剂10%的福尔马林处理。石蜡切片用苏木精-伊红染色。细胞核测量分析在3000倍放大倍数下进行，每个样本测量200个细胞。描述了室旁核、弓状核区域以及第三脑室室管膜中神经分泌细胞的变化。室旁核和弓状核的细胞核测量分析结果表明，注射Gn-RH以及下丘脑-垂体区域照射和卵巢直接照射，均刺激了所研究的脑结构。注射Gn-RH并随后照射下丘脑-垂体区域后，第三脑室室管膜观察到的变化不显著；只有在卵巢直接照射后，这些细胞才通过反馈机制受到间接作用的抑制。
