噬菌体SPO1 DNA聚合酶与病毒基因31的活性

Bacteriophage SPO1 DNA polymerase and the activity of viral gene 31.

作者信息

De Antoni G L, Besso N E, Zanassi G E, Sarachu A N, Grau O

出版信息

Virology. 1985 May;143(1):16-22. doi: 10.1016/0042-6822(85)90092-3.

Abstract

Bacteriophage SPO1 DNA-negative (D0) mutants were tested for the induction of viral DNA polymerase during Bacillus subtilis infection. Extracts from SPO1-infected bacteria exhibited enzymatic activity when representative mutants of seven out of the nine known D0 genes were employed. This activity was undetectable in cells infected with mutants in genes 28 and 31. The product of gene 28 (gp28) is known to be responsible for turning on SPO1 middle gene expression. Results show that nonsense mutation in gene 31 leads to the absence of a single polypeptide of 100-105 kDa and that phage DNA synthesis "in vivo" directly depends on gp31 activity. Based on these data it is proposed that SPO1 gene 31 codes for the viral DNA polymerase.

摘要

对噬菌体SPO1 DNA阴性（D0）突变体进行了测试，以检测其在枯草芽孢杆菌感染期间对病毒DNA聚合酶的诱导作用。当使用九个已知D0基因中的七个基因的代表性突变体时，来自SPO1感染细菌的提取物表现出酶活性。在感染基因28和31突变体的细胞中未检测到这种活性。已知基因28的产物（gp28）负责开启SPO1中期基因表达。结果表明，基因31中的无义突变导致缺少一条100 - 105 kDa的单一多肽，并且噬菌体DNA在“体内”的合成直接依赖于gp31的活性。基于这些数据，有人提出SPO1基因31编码病毒DNA聚合酶。