Tu Yuhan, Ning Yangyang, Wang Hong, Li Yang, Yao Zhiang, Tao Shanhui, Yang Wenjing, Li Bin, Li Xiaofen, He Huacheng, Li Shijun
Institute of Life Sciences, Wenzhou University, Wenzhou 325035, PR China; Department of Pharmacy, Yueqing third people's hospital, Wenzhou 325604, PR China; Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision, and Brain Health), School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou 325035, PR China.
Institute of Life Sciences, Wenzhou University, Wenzhou 325035, PR China.
Colloids Surf B Biointerfaces. 2025 Jan;245:114263. doi: 10.1016/j.colsurfb.2024.114263. Epub 2024 Sep 25.
Rapid and scar-free healing of burn wounds is an urgent clinical issue. Basic fibroblast growth factor (bFGF) has been proven to promote the healing of burn wounds by accelerating ECM remodeling and angiogenesis. However, exudates from burn wounds can accelerate bFGF degradation, thereby affecting its bioactivity. This study proposes an effective protection strategy for bFGF that involves encapsulating bFGF in nanoliposomes (bFGF-NLip) and then incorporating bFGF-NLip into a bovine serum albumin (BSA) hydrogel. This hybrid hydrogel system (bFGF-NLip@B) could maintain the activity of bFGF, achieve sustained release, and allow phospholipids and cholesterol to penetrate the skin, thereby enabling bFGF to function in the dermis. The experimental results showed that the hydrogel was injectable with good mechanical properties and biocompatibility. In a mouse scald wound model, owing to the sustained release of bFGF and skin permeation function of the nanoliposomes, the hydrogel promoted granulation formation, collagen deposition, vascular regeneration, and re-epithelialisation, ultimately accelerating wound healing. In addition, the hydrogel effectively inhibited scar formation. This system provides novel insights into the delivery of bFGF and scar-free healing of burn wounds.
烧伤创面的快速无瘢痕愈合是一个亟待解决的临床问题。碱性成纤维细胞生长因子（bFGF）已被证明可通过加速细胞外基质重塑和血管生成来促进烧伤创面愈合。然而，烧伤创面的渗出物会加速bFGF降解，从而影响其生物活性。本研究提出了一种针对bFGF的有效保护策略，即将bFGF包裹在纳米脂质体（bFGF-NLip）中，然后将bFGF-NLip掺入牛血清白蛋白（BSA）水凝胶中。这种混合水凝胶系统（bFGF-NLip@B）可以保持bFGF的活性，实现持续释放，并使磷脂和胆固醇穿透皮肤，从而使bFGF在真皮中发挥作用。实验结果表明，该水凝胶具有可注射性，机械性能和生物相容性良好。在小鼠烫伤创面模型中，由于bFGF的持续释放和纳米脂质体的皮肤渗透功能，该水凝胶促进了肉芽组织形成、胶原蛋白沉积、血管再生和上皮再形成，最终加速了创面愈合。此外，该水凝胶有效抑制了瘢痕形成。该系统为bFGF的递送和烧伤创面的无瘢痕愈合提供了新的见解。
