Chen Haojiao, Sun Jianping, Liu Wenyang
Department of Burn Plastic and wound Repair, Shaoxing Second Hospital, Shaoxing, China.
J Biomater Appl. 2025 May;39(10):1156-1164. doi: 10.1177/08853282251321943. Epub 2025 Feb 18.
Diabetic wound is one of the most common diabetic chronic complications. Effective treatments of diabetic wound remain limited. Here, we explored the effects of basic fibroblast growth factor (bFGF)-acellular dermal matrix (ADM) hydrogel on the diabetic wound. The bFGF-ADM hydrogel was manufactured by mixing 180 µL ADM hydrogel and 20 µL bFGF aqueous solution (10 mg/mL). The morphology of ADM hydrogel and bFGF-ADM hydrogel was observed under scanning electron microscope. The release property of bFGF-ADM hydrogel was determined by ELISA. CCK-8 assay was utilized to estimate the cell viability of mouse skin fibroblasts. The diabetes mellitus (DM) model was established in rats. The four wounds on the back of each DM rat were treated with the ADM hydrogel, bFGF-ADM hydrogel, bFGF aqueous solution and no solution (control), respectively. The wound healing rate of each rat was estimated. The traumatized skin tissue of each rat was observed by H&E staining and Sirius red staining. The bFGF-ADM hydrogel displayed an interconnected pore structure and bFGF was gradually released from the bFGF-ADM hydrogel over time. The bFGF-ADM hydrogel could enhance the cell viability of skin fibroblasts and promote the wound healing rate, the re-epithelialization of wound and increase the collagen fiber content of dermis. And the bFGF-ADM hydrogel exhibited better therapeutic effects of diabetic wound than either bFGF or ADM alone. Our study revealed that the bFGF-ADM hydrogel could promote diabetic wound healing.
糖尿病伤口是最常见的糖尿病慢性并发症之一。糖尿病伤口的有效治疗方法仍然有限。在此，我们探讨了碱性成纤维细胞生长因子（bFGF）-无细胞真皮基质（ADM）水凝胶对糖尿病伤口的影响。bFGF-ADM水凝胶通过将180µL ADM水凝胶与20µL bFGF水溶液（10mg/mL）混合制成。在扫描电子显微镜下观察ADM水凝胶和bFGF-ADM水凝胶的形态。通过酶联免疫吸附测定法测定bFGF-ADM水凝胶的释放特性。采用CCK-8法评估小鼠皮肤成纤维细胞的细胞活力。在大鼠中建立糖尿病（DM）模型。每只DM大鼠背部的四个伤口分别用ADM水凝胶、bFGF-ADM水凝胶、bFGF水溶液和不使用任何溶液（对照）进行处理。评估每只大鼠的伤口愈合率。通过苏木精-伊红染色和天狼星红染色观察每只大鼠的创伤皮肤组织。bFGF-ADM水凝胶呈现出相互连接的孔隙结构，并且随着时间的推移bFGF从bFGF-ADM水凝胶中逐渐释放。bFGF-ADM水凝胶可增强皮肤成纤维细胞的细胞活力，促进伤口愈合率、伤口的再上皮化，并增加真皮的胶原纤维含量。并且bFGF-ADM水凝胶对糖尿病伤口的治疗效果比单独使用bFGF或ADM更好。我们的研究表明，bFGF-ADM水凝胶可促进糖尿病伤口愈合。
