College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Plant Physiol Biochem. 2024 Nov;216:109148. doi: 10.1016/j.plaphy.2024.109148. Epub 2024 Sep 24.
Tillers are specialized lateral shoots arising from axillary buds at basal nodes, and are also an important agronomic trait that determines the aboveground biomass and grain yield of various gramineous crops. So far, few genes have been reported to control tiller formation and most have been in the annual crop rice (Oryza sativa). Orchardgrass (Dactylis glomerata) is an important perennial forage crop with great economic and ecological value, but its genes regulating tillering have remained largely unknown. In the present study, we used a natural population of 264 global orchardgrass germplasms to determine genes associated with quantitative variation in tiller number through genome-wide association study analysis. A total of 19 putative loci and 55 genes associated with tiller number were thus identified. Additionally, 26 putative differentially expressed genes with tiller number, including DgCYC-C1, were identified by RNA-seq and genome-wide association study analysis. DgCYC-C1 which is involved in cell division, was overexpressed, revealing that DgCYC-C1 positively regulates tiller number. These results provide some new candidate genes or loci for the improvement of tiller number in crops, which might advance new sustainable strategies to meet global crop production challenges.
分蘖是从基部节腋芽生出的特化侧枝，也是决定各种禾本科作物地上生物量和籽粒产量的重要农艺性状。迄今为止，已经报道了少数几个控制分蘖形成的基因，而且大多数都在一年生作物水稻（Oryza sativa）中。多年生牧草黑麦草（Dactylis glomerata）是一种具有重要经济和生态价值的重要牧草作物，但调节分蘖的基因在很大程度上仍不清楚。本研究利用 264 个全球黑麦草种质的自然群体，通过全基因组关联研究分析，确定与分蘖数数量变化相关的基因。共鉴定出 19 个与分蘖数相关的假定基因座和 55 个基因。此外，通过 RNA-seq 和全基因组关联研究分析，还鉴定出了 26 个与分蘖数相关的假定差异表达基因，包括 DgCYC-C1。DgCYC-C1 参与细胞分裂，过量表达，表明 DgCYC-C1 正向调节分蘖数。这些结果为提高作物分蘖数提供了一些新的候选基因或基因座，可能为应对全球作物生产挑战提供新的可持续策略。
