College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Int J Mol Sci. 2023 Oct 30;24(21):15762. doi: 10.3390/ijms242115762.
Tillering is a special type of branching and one of the important contributors to the yield of cereal crops. Strigolactone and sucrose play a vital role in controlling tiller formation, but their mechanism has not been elucidated completely in most crops. Orchardgrass ( L.) is an important perennial forage with prominent tillering ability among crops. To date, the mechanism of tillering in orchardgrass is still largely unknown. Therefore, we performed a transcriptome and miRNA analysis to reveal the potential RNA mechanism of tiller formation under strigolactone and sucrose treatment in orchardgrass. Our results found that , , , , miRNA4393-z, and miRNA531-z could be key factors to control tiller bud development in orchardgrass. In addition, strigolactones might affect the ABA biosynthesis pathway to regulate the tiller bud development of orchardgrass, which may be related to the expression changes in miRNA4393-z, , and . miRNA531-z could be involved in the interaction of strigolactones and sucrose in regulating tillering. These results will be further used to clarify the potential mechanism of tillering for breeding new high-tillering and high-production orchardgrass varieties and beneficial to improving the production and reproduction of crops.
分蘖是一种特殊的分枝方式，是谷类作物产量的重要贡献因素之一。独脚金内酯和蔗糖在控制分蘖形成中起着至关重要的作用，但它们在大多数作物中的作用机制尚未完全阐明。梯牧草（Lolium perenne）是一种重要的多年生饲料作物，具有突出的分蘖能力。迄今为止，梯牧草分蘖的机制在很大程度上仍不清楚。因此，我们进行了转录组和 miRNA 分析，以揭示独脚金内酯和蔗糖处理下梯牧草分蘖形成的潜在 RNA 机制。我们的结果发现，miR156f，miR164a，miR396a，miR396b，miR4393-z 和 miR531-z 可能是控制梯牧草分蘖芽发育的关键因素。此外，独脚金内酯可能通过影响 ABA 生物合成途径来调节梯牧草分蘖芽的发育，这可能与 miR4393-z、miR167a 和 miR396a 的表达变化有关。miR531-z 可能参与独脚金内酯和蔗糖调节分蘖的相互作用。这些结果将进一步用于阐明分蘖的潜在机制，以培育新的高分蘖、高产梯牧草品种，并有助于提高作物的产量和繁殖能力。
