正常血细胞中α-甘露糖苷酶、α-岩藻糖苷酶和中性麦芽糖酶的细胞化学模式

Cytochemical pattern of alpha-mannosidase, alpha-fucosidase and neutral maltase in normal blood cells.

作者信息

Nano R, Gerzeli G, Invernizzi R, Perseghin P

出版信息

Acta Histochem. 1985;77(1):55-9. doi: 10.1016/s0065-1281(85)80015-5.

Abstract

The activity of alpha-mannosidase, alpha-fucosidase and neutral maltase was studied by cytochemical techniques in blood cells of 20 controls and of 4 T and B lymphocyte concentrates. All granulocytes, monocytes and platelets showed fine or coarse reaction product deposition, whereas lymphocytes were negative or showed various positivity patterns. A significant difference of the positivity between T and B subpopulations was observed only for the fucosidase reaction. It is possible that the different positivity patterns of the lymphoid cells are related to different functional activities. Further studies will probably confirm the interest of the alpha-fucosidase reaction for the characterization of normal and pathological lymphoid cells.

摘要

采用细胞化学技术研究了20名对照者以及4份T和B淋巴细胞浓缩物血细胞中α-甘露糖苷酶、α-岩藻糖苷酶和中性麦芽糖酶的活性。所有粒细胞、单核细胞和血小板均显示出细颗粒或粗颗粒反应产物沉积，而淋巴细胞呈阴性或显示出不同的阳性模式。仅在岩藻糖苷酶反应中观察到T和B亚群之间的阳性存在显著差异。淋巴细胞不同的阳性模式可能与不同的功能活性有关。进一步的研究可能会证实α-岩藻糖苷酶反应对于正常和病理性淋巴细胞特征鉴定的意义。