Uri J V
Acta Microbiol Hung. 1985;32(2):133-45.
The production or presence of beta-lactamase(s) was studied by the rapid method utilizing the chromogenic cephalosporin compound nitrocefin in cultures of multiple strains belonging to the same genus as well as groups of microorganisms. The genera were: Staphylococcus spp., Streptococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, indole-positive Proteus spp., Enterobacter spp., Serratia marcescens, rare Enterobacteriaceae, Pseudomonas aeruginosa, Haemophilus influenzae and Neisseria gonorrhoeae. With this sensitive and rapid assay for beta-lactamase, it was possible to verify and separate the beta-lactamase producing cultures from the non-producers and include the useful strains to on-going research, such as beta-lactam screen, beta-lactamase inhibitory study and lytic properties of beta-lactams. The data also provide evidence for the possible role of beta-lactamase(s) in the physiology, biochemistry and pathogenicity of bacterial strains. The nitrocefin method was found a very specific and extremely useful procedure for the detection and estimation of beta-lactamase activity.
利用生色头孢菌素化合物硝基头孢菌素的快速方法，对属于同一属的多个菌株以及微生物群体的培养物中的β-内酰胺酶的产生或存在情况进行了研究。这些属包括：葡萄球菌属、粪肠球菌、大肠杆菌、肺炎克雷伯菌、奇异变形杆菌、吲哚阳性变形杆菌属、肠杆菌属、粘质沙雷氏菌、罕见肠杆菌科细菌、铜绿假单胞菌、流感嗜血杆菌和淋病奈瑟菌。通过这种对β-内酰胺酶敏感且快速的检测方法，能够验证并区分产生β-内酰胺酶的培养物和不产生β-内酰胺酶的培养物，并将有用的菌株纳入正在进行的研究中，如β-内酰胺筛选、β-内酰胺酶抑制研究以及β-内酰胺的裂解特性研究。这些数据也为β-内酰胺酶在细菌菌株的生理学、生物化学和致病性中可能发挥的作用提供了证据。发现硝基头孢菌素方法是检测和评估β-内酰胺酶活性的一种非常特异且极其有用的方法。
