Bibikova M V, Ivanitskaia L P, Nikishina V G, Kuznetsova S M
Antibiotiki. 1983 Aug;28(8):566-73.
Fifty clinical strains of Gram-negative bacteria highly resistant to beta-lactam antibiotics were studied and 9 strains producing beta-lactamases were selected. From these cultures crude beta-lactamases were isolated. The substrate specificity of these crude preparations and their sensitivity to the inhibitory effect of dicloxacillin, methicillin and carbenicillin were studied with the spectrophotometric and hydroxylamine methods. The results of the study obtained with the 2 methods were in the main comparable. 5 crude preparations had the properties of broad-spectrum beta-lactamases without pronounced selectivity with respect to any beta-lactamide. However, they differed in the level of inhibition by dicloxacillin, methicillin and carbenicillin. 4 crude preparations with a pronounced substrate profile were recommended for use in screening and investigation of natural inhibitors of beta-lactamases and beta-lactam antibiotics. These 4 preparations were cephalosporinase from Enterobacter cloaceae 975, oxacillinase from Pseudomonas aeruginosa 10380, carbenicillinase from Pseudomonas aeruginosa 10538 and beta-lactamase of the TEM type from Enterobacter cloaceae 980.
对50株对β-内酰胺抗生素高度耐药的革兰氏阴性菌临床菌株进行了研究，并挑选出9株产β-内酰胺酶的菌株。从这些培养物中分离出粗制β-内酰胺酶。采用分光光度法和羟胺法研究了这些粗制品的底物特异性及其对双氯西林、甲氧西林和羧苄西林抑制作用的敏感性。用这两种方法获得的研究结果基本可比。5种粗制品具有广谱β-内酰胺酶的特性，对任何β-内酰胺均无明显选择性。然而，它们在双氯西林、甲氧西林和羧苄西林的抑制水平上有所不同。推荐4种具有明显底物谱的粗制品用于筛选和研究β-内酰胺酶的天然抑制剂及β-内酰胺抗生素。这4种制品分别是阴沟肠杆菌975的头孢菌素酶、铜绿假单胞菌10380的苯唑西林酶、铜绿假单胞菌10538的羧苄西林酶以及阴沟肠杆菌980的TEM型β-内酰胺酶。
