Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong, P. R. of China.
School of Earth Sciences, Zhejiang University, Hangzhou, Zhejiang, P. R. of China.
Sci Data. 2024 Sep 27;11(1):1043. doi: 10.1038/s41597-024-03889-7.
Coastal sediments are rich in embedded recalcitrant organic carbons that are biotransformed into methane. In this study, gas composition (carbon dioxide, methane and nitrogen) and chemical indicators (total nitrogen, total carbon, and total sulfate) were examined in five deep sediment cores (up to 130 m in length) obtained from the Hangzhou Bay. The V3-V4 region of the 16S rRNA gene amplicons was amplified and sequenced for the prokaryotic community analysis. The species composition, along with the physicochemical factors of the sediments, revealed a strong correlation with methane content in one of the sediment cores. We then obtained metagenomes of the two sediment samples selected for their high methane content and enrichment of methanogenic Bathyarchaeota with phylogenetic evidence. A total of 27 draft genomes were retrieved through metagenomic binning methodologies and were classified into Bathyarchaeota, Asgard archaea, Planctomycetes, and other microbial groups. The data provided are valuable for understanding the relationship between methane generation and microbial community composition in deep sediment core samples from coastal to marine environments.
沿海沉积物中富含嵌入的难降解有机碳，这些有机碳会被生物转化为甲烷。在这项研究中，对取自杭州湾的五个深沉积岩芯（最长达 130 米）中的气体组成（二氧化碳、甲烷和氮气）和化学指标（总氮、总碳和总硫酸盐）进行了检查。对 16S rRNA 基因扩增子的 V3-V4 区进行了扩增和测序，以进行原核生物群落分析。物种组成以及沉积物的理化因素与其中一个沉积物芯中甲烷含量之间存在很强的相关性。然后，我们从两个选择的甲烷含量高且富含甲烷生成菌 Bathyarchaeota 的沉积物样本中获得了宏基因组，这些样本有系统发育证据支持。通过宏基因组 binning 方法共获得了 27 个草案基因组，并将其分类为 Bathyarchaeota、Asgard 古菌、Planctomycetes 和其他微生物群。这些数据对于了解从沿海到海洋环境的深沉积岩芯样本中甲烷生成与微生物群落组成之间的关系具有重要价值。
