Liu Wenhui, Zou Huren, You Danming, Zhang Huijie, Xu Lingling
Department of Endocrinology, Shenzhen Hospital, Southern Medical University, Shenzhen 518000, China.
Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou 510000, China.
Antioxidants (Basel). 2024 Aug 30;13(9):1057. doi: 10.3390/antiox13091057.
The rising prevalence of obesity has resulted in an increased demand for innovative and effective treatment strategies. Thunb. has demonstrated promising potential in preventing obesity. However, the mechanism underlying the anti-obesity effects of and its bioactive component, sodium houttuybonate (SH), remains unclear. Our study reveals that SH treatment promotes the browning of inguinal white adipose tissue (iWAT) and prevents the obesity induced by a high-fat diet. SH significantly mitigates ferroptosis by upregulating glutathione peroxidase 4 () and decreasing malondialdehyde (MDA) levels, while also enhancing superoxide dismutase (SOD) levels. Furthermore, SH promotes the phosphorylation of AMP-activated protein kinase (AMPK), which subsequently increases the expression of nuclear factor erythroid 2-related factor 2 (NRF2) and heme oxygenase-1 (HO-1) in the iWAT. However, the effects of SH were attenuated by ML385, an Nrf2 inhibitor. Collectively, our findings suggest that SH induces iWAT browning and prevents diet-induced obesity primarily through the AMPK/NRF2/HO-1 pathway by inhibiting ferroptosis.
肥胖患病率的不断上升导致对创新且有效的治疗策略的需求增加。土荆芥已在预防肥胖方面展现出了有前景的潜力。然而，土荆芥及其生物活性成分鱼腥草素钠（SH）的抗肥胖作用机制仍不清楚。我们的研究表明，SH处理可促进腹股沟白色脂肪组织（iWAT）的褐变，并预防高脂饮食诱导的肥胖。SH通过上调谷胱甘肽过氧化物酶4（）和降低丙二醛（MDA）水平，同时还提高超氧化物歧化酶（SOD）水平，显著减轻铁死亡。此外，SH促进AMP活化蛋白激酶（AMPK）的磷酸化，随后增加iWAT中核因子红细胞2相关因子2（NRF2）和血红素加氧酶-1（HO-1）的表达。然而，SH的作用被Nrf2抑制剂ML385减弱。总体而言，我们的研究结果表明，SH主要通过抑制铁死亡，经由AMPK/NRF2/HO-1途径诱导iWAT褐变并预防饮食诱导的肥胖。
