羊肚菌子实体多糖的分离纯化、结构特征及免疫调节活性研究。
Purification, structural characterization and immunomodulatory activities of a polysaccharide from the fruiting body of Morchella sextelata.
机构信息
State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
出版信息
Int J Biol Macromol. 2022 Jul 31;213:394-403. doi: 10.1016/j.ijbiomac.2022.05.096. Epub 2022 May 16.
A novel polysaccharide (MSP-1) was isolated from the fruiting body of Morchella sextelata and purified using DEAE-52 and Sephadex G-75. The molecular weight of MSP-1 was 1.17 × 10 Da, as detected by HPLC analysis. The monosaccharide composition of MSP-1 was mannose and glucose at a ratio of 1.00: 1.25. Methylation and NMR results revealed that the backbone of MSP-1 was composed of →4)-β-D-Manp-(1→, →4)-β-D-Glcp-(1→, →4)-α-D-Glcp-(1→, and →4, 6)-α-D-Glcp-(1→. SEM images of MSP-1 presented a dense network structure with porous characterizations. The immunomodulatory activities of MSP-1 were evaluated using RAW264.7 cells, and the results showed that MSP-1 promoted proliferative and phagocytic activity and increased the production of NO, TNF-α and IL-6. These results indicated that MSP-1 exhibited significant immunomodulatory activities.
从羊肚菌子实体中分离得到一种新型多糖（MSP-1），并使用 DEAE-52 和 Sephadex G-75 进行纯化。HPLC 分析表明，MSP-1 的分子量为 1.17×10 Da。MSP-1 的单糖组成是甘露糖和葡萄糖，比例为 1.00:1.25。甲基化和 NMR 结果表明，MSP-1 的骨架由→4)-β-D-Manp-(1→、→4)-β-D-Glcp-(1→、→4)-α-D-Glcp-(1→和→4,6)-α-D-Glcp-(1→组成。MSP-1 的 SEM 图像呈现出密集的网络结构和多孔特征。使用 RAW264.7 细胞评估了 MSP-1 的免疫调节活性，结果表明 MSP-1 促进了细胞增殖和吞噬活性，并增加了 NO、TNF-α 和 IL-6 的产生。这些结果表明 MSP-1 具有显著的免疫调节活性。
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