Boggan SaraBeth, Awosile Babafela, Koziol Jennifer
School of Veterinary Medicine, Texas Tech University, 7671 Evans Drive, Amarillo, TX 79106, USA.
Animals (Basel). 2024 Sep 16;14(18):2689. doi: 10.3390/ani14182689.
, the causative agent of bovine trichomoniasis, is an obligate protozoan parasite of the bovine reproductive tract and can be found on the penis, prepuce, and distal urethra of the bull and from the cranial vagina to the oviduct in the infected cow. To date, the microbiome of bulls infected with has not been described. The objectives of this study were to (1) describe the preputial and penile microbiome of bulls chronically infected by (2) describe the seminal microbiome of -infected bulls, and (3) evaluate different collection devices that could be used for sampling. Eleven bulls naturally infected with were utilized for the collection of samples. Samples were obtained during the process of a routine breeding soundness exam utilizing either a dacron swab, pizzle stick, double-guarded swab, or semen collection. The preputial and seminal microbiome of -infected bulls was dominated by bacterial members of the phyla , and Semen collection yielded the most microbial diversity; however, there was no significant difference between the four methods ( ≥ 0.05). This study characterizes both the preputial and seminal microbial communities of bulls chronically infected by
牛毛滴虫病的病原体，是牛生殖道专性原生动物寄生虫，可在公牛的阴茎、包皮和尿道远端以及感染母牛的从阴道前端到输卵管中发现。迄今为止，感染该病原体的公牛的微生物群尚未被描述。本研究的目的是：（1）描述长期感染该病原体的公牛的包皮和阴茎微生物群；（2）描述感染该病原体的公牛的精液微生物群；（3）评估可用于采样的不同采集装置。11头自然感染该病原体的公牛用于样本采集。样本是在常规繁殖健康检查过程中，使用涤纶拭子、阴茎探子、双保护拭子或精液采集法获得的。感染该病原体的公牛的包皮和精液微生物群以厚壁菌门、变形菌门和放线菌门的细菌成员为主。精液采集产生的微生物多样性最高；然而，四种方法之间没有显著差异（P≥0.05）。本研究描述了长期感染该病原体的公牛的包皮和精液微生物群落特征 。
