Irons P C, Henton M M, Bertschinger H J
Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
J S Afr Vet Assoc. 2002 Jun;73(2):66-9. doi: 10.4102/jsava.v73i2.558.
Two trials were carried out to assess the diagnostic sensitivity and practicability of preputial scraping as a method of collecting preputial material from bulls infected with Tritrichomonas foetus. In the 1st trial, preputial material was collected by simultaneous scraping and aspiration from 3 infected and 1 uninfected bull 10 times over a 5-week period. In the 2nd trial, samples from 5 infected bulls were collected by both sheath washing and scraping on 6 occasions, while 8 uninfected animals were sampled 3 times. Samples were cultured using a modified Trichomonas culture medium (Oxoid). In the first trial, 29 of 30 samples from infected bulls were found to be positive. In the second trial, 83 % of samples collected by both methods tested positive. In neither trial were any samples from the control bulls found to be positive. Scraping was found to be quick and safe, and offered advantages over preputial washing in that urine contamination was easily avoided, samples were smaller and more concentrated and contamination was reduced. It may, however, be subject to greater operator variability than sheath washing. It is concluded that preputial scraping is as effective as washing and represents a suitable alternative for the collection of material for direct examination and culture of Tritrichomonas foetus.
进行了两项试验，以评估包皮刮片作为从感染胎儿三毛滴虫的公牛收集包皮材料的一种方法的诊断敏感性和实用性。在第一项试验中，在5周的时间内，对3头感染公牛和1头未感染公牛同时进行刮片和抽吸，共收集10次包皮材料。在第二项试验中，对5头感染公牛的样本进行了6次鞘冲洗和刮片采集，同时对8头未感染动物进行了3次采样。样本使用改良的滴虫培养基（Oxoid）进行培养。在第一项试验中，感染公牛的30个样本中有29个呈阳性。在第二项试验中，两种方法采集的样本中有83%检测呈阳性。在两项试验中，对照公牛的任何样本均未发现呈阳性。发现刮片快速且安全，与包皮冲洗相比具有优势，因为易于避免尿液污染，样本更小且更浓缩，污染减少。然而，与鞘冲洗相比，它可能受操作人员变异性的影响更大。得出的结论是，包皮刮片与冲洗一样有效，是收集用于直接检查和培养胎儿三毛滴虫的材料的合适替代方法。
