Cao Xiukai, Ling Chen, Liu Yongqi, Gu Yifei, Huang Jinlin, Sun Wei
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Animals (Basel). 2024 Sep 20;14(18):2721. doi: 10.3390/ani14182721.
Uncovering genes associated with muscle growth and body size will benefit the molecular breeding of meat Hu sheep. has proven to be an important gene in mouse muscle growth and is associated with the body size of various species. However, its roles in sheep are still limited. Using sheep myoblast as a cell model, the overexpression of significantly promoted sheep myoblast proliferation, while interference with expression inhibited proliferation, indicated by qPCR, EdU, and CCK-8 assays. Furthermore, the dual-luciferase reporter system indicated that the region NC_056056.1: 154134300-154134882 (-618 to -1200 bp upstream of the transcription start site) was one of the habitats of the core promoter, given the observation that this fragment led to a ~3-fold increase in luciferase activity. Interestingly, SNP rs428001129 (NC_056056.1:g.154134315 C>A) was detected in this fragment by Sanger sequencing of the PCR product of pooled DNA from 458 crossbred sheep. This SNP was found to affect the promoter activity and was significantly associated with chest width at birth and two months old, as well as chest depth at two and six months old. The data obtained in this study demonstrated the phenotypic regulatory role of the gene in sheep production traits and the potential of rs428001129 in marker-assisted selection for sheep breeding in terms of chest width and chest depth.
揭示与肌肉生长和体型相关的基因将有利于肉用湖羊的分子育种。 已被证明是小鼠肌肉生长中的一个重要基因，并且与各种物种的体型有关。然而，它在绵羊中的作用仍然有限。以绵羊成肌细胞为细胞模型， 通过qPCR、EdU和CCK-8检测表明， 的过表达显著促进了绵羊成肌细胞的增殖，而干扰 表达则抑制了增殖。此外，双荧光素酶报告系统表明，区域NC_056056.1: 154134300-154134882（ 转录起始位点上游-618至-1200 bp）是 核心启动子的栖息地之一，因为观察到该片段导致荧光素酶活性增加了约3倍。有趣的是，通过对458只杂交绵羊混合DNA的PCR产物进行Sanger测序，在该片段中检测到SNP rs428001129（NC_056056.1:g.154134315 C>A）。发现该SNP影响启动子活性，并且与出生时和两个月大时的胸宽以及两个月和六个月大时的胸深显著相关。本研究获得的数据证明了 基因在绵羊生产性状中的表型调控作用，以及rs428001129在绵羊育种中胸宽和胸深标记辅助选择方面的潜力。
