Rutkowska Elżbieta, Kwiecień Iwona, Raniszewska Agata, Sokołowski Rafał, Bednarek Joanna, Jahnz-Różyk Karina, Chciałowski Andrzej, Rzepecki Piotr
Laboratory of Hematology and Flow Cytometry, Department of Internal Medicine and Hematology, Military Institute of Medicine-National Research Institute, 04-141 Warsaw, Poland.
Department of Internal Medicine, Pneumonology, Allergology, Clinical Immunology and Rare Diseases, 04-141 Warsaw, Poland.
Biomedicines. 2024 Sep 4;12(9):2016. doi: 10.3390/biomedicines12092016.
The neutrophils evaluation seems interesting in the initial qualifications of patients with various inflammatory processes. In this study, we presented analysis of neutrophils and new parameters of the complexity (NEUT-GI, NE-WX), maturation (IG), size (NE-FSC, NE-WZ), and neutrophil activities (NEUT-RI, NE-WY) in coronavirus disease 2019 (COVID-19), lung cancer (LC), sarcoidosis (SA), and healthy controls (HCs). Peripheral blood (PB) was collected. The new parameters were examined by the Sysmex XN-1500. The mean absolute value for the IG parameter was the highest in the LC group. The differences in NEUT-RI value between COVID-19 and the HC group were observed. No significant differences were noticed between groups in the NEUT-GI granularity parameter. Neutrophil size assessed by NE-FSC parameter was reduced in all groups compared to HCs. The values of complexity (NE-WX), fluorescence (NE-WY), and size (NE-WZ) were the lowest in the HCs, whereas the highest median proportions of NE-WX, NE-WY, and NE-WZ were in LC patients. Patients from the SA group differed significantly from the HC group only for the NE-WZ parameter. We showed the usefulness of neutrophil parameters and their reactivity, morphology, and exhaustion. A more detailed analysis of blood counts may reveal trends that indicate a disease-specific immune response.
在对患有各种炎症过程的患者进行初步评估时，中性粒细胞评估似乎很有意义。在本研究中，我们分析了2019冠状病毒病（COVID-19）、肺癌（LC）、结节病（SA）和健康对照（HC）中中性粒细胞及其复杂性（NE-GI、NE-WX）、成熟度（IG）、大小（NE-FSC、NE-WZ）和中性粒细胞活性（NEUT-RI、NE-WY）的新参数。采集外周血（PB）。通过Sysmex XN-1500检测新参数。LC组中IG参数的平均绝对值最高。观察到COVID-19组与HC组之间NEUT-RI值的差异。在NEUT-GI颗粒度参数方面，各小组之间未发现显著差异。与HC组相比，所有组中通过NE-FSC参数评估的中性粒细胞大小均减小。HC组中复杂性（NE-WX）、荧光（NE-WY）和大小（NE-WZ）的值最低，而LC患者中NE-WX、NE-WY和NE-WZ的中位数比例最高。SA组患者仅在NE-WZ参数方面与HC组有显著差异。我们展示了中性粒细胞参数及其反应性、形态和耗竭的有用性。对血细胞计数进行更详细的分析可能会揭示表明疾病特异性免疫反应的趋势。
