He Hongyan, Yang Xiuwen, Zeb Aurang, Liu Jiasi, Gu Huiyue, Yang Jieru, Xiang Wenyu, Shen Songdong
School of Biology & Basic Medical Sciences, Soochow University, Suzhou 215101, China.
Suzhou Industrial Park Environmental Law Enforcement Brigade, Suzhou 215021, China.
Biology (Basel). 2024 Sep 5;13(9):695. doi: 10.3390/biology13090695.
The xanthophyll cycle is a photoprotective mechanism in plants and algae, which protects the photosynthetic system from excess light damage under abiotic stress. Zeaxanthin is considered to play a pivotal role in this process. In this study, the relative content of xanthophylls was determined using HPLC-MS/MS in exposed to different salinities. The results showed that high-salt stress significantly increased the relative content of xanthophylls and led to the accumulation of zeaxanthin. It was speculated that the accumulated zeaxanthin may contribute to the response of to high-salt stress. Zeaxanthin epoxidase () is a key enzyme in the xanthophyll cycle and is also involved in the synthesis of abscisic acid and carotenoids. In order to explore the biological function of , a gene was cloned and identified from . The CDS of is 1122 bp and encodes 373 amino acids. Phylogenetic analysis showed that clusters within a clade of green algae. The results of qRT-PCR showed that high-salt stress induced the expression of . In addition, heterologous overexpression of the gene in yeast and improved the salt tolerance of transgenic organisms. In conclusion, the gene may be involved in the response of to high-salt stress and can improve the high-salt tolerance of transgenic organisms.
叶黄素循环是植物和藻类中的一种光保护机制，可在非生物胁迫下保护光合系统免受过量光照的损害。玉米黄质被认为在这一过程中起关键作用。在本研究中，使用高效液相色谱-串联质谱法（HPLC-MS/MS）测定了暴露于不同盐度下的叶黄素相对含量。结果表明，高盐胁迫显著增加了叶黄素的相对含量，并导致玉米黄质的积累。据推测，积累的玉米黄质可能有助于[具体对象]对高盐胁迫的响应。玉米黄质环氧化酶（ZEP）是叶黄素循环中的关键酶，也参与脱落酸和类胡萝卜素的合成。为了探究ZEP的生物学功能，从[具体对象]中克隆并鉴定了一个ZEP基因。该ZEP的编码序列（CDS）为1122 bp，编码373个氨基酸。系统发育分析表明，ZEP聚类在绿藻的一个进化枝内。实时定量聚合酶链反应（qRT-PCR）结果表明，高盐胁迫诱导了ZEP的表达。此外，ZEP基因在酵母和[具体对象]中的异源过表达提高了转基因生物的耐盐性。总之，ZEP基因可能参与了[具体对象]对高盐胁迫的响应，并能提高转基因生物的高盐耐受性。
