Noh Eun-Soo, Shin Eun-Ha, Kong Hee-Jeong, Kim Young-Ok, Ryu Yong-Woon
Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea.
Subtropical Fisheries Research Institute, National Institute of Fisheries Science, Jeju 63610, Republic of Korea.
Biology (Basel). 2024 Sep 11;13(9):710. doi: 10.3390/biology13090710.
(1) Background: The small yellow croaker, an economically important fish in East Asia, has been subjected to population declines due to overfishing and environmental pressures. The development of effective breeding programs is considered crucial for the species, and accurate parentage assignment is deemed essential for such programs. (2) Methods: The assembled reference genome of the small yellow croaker was utilized to select highly polymorphic microsatellite markers. A multiplex PCR system was optimized for the simultaneous amplification of these markers. The system's accuracy was validated using controlled mating pairs and subsequently applied to a group mating scenario. (3) Results: The developed multiplex PCR system demonstrated high accuracy in assigning offspring to their parents in both the controlled and group mating scenarios. (4) Conclusions: The system is presented as a valuable tool for pedigree management, selective breeding, and conservation efforts for the small yellow croaker, facilitating sustainable aquaculture practices and genetic improvement.
（1）背景：小黄鱼是东亚一种具有重要经济价值的鱼类，由于过度捕捞和环境压力，其种群数量一直在下降。有效的育种计划的制定被认为对该物种至关重要，而准确的亲权鉴定被认为是此类计划必不可少的。（2）方法：利用组装好的小黄鱼参考基因组选择高度多态性的微卫星标记。优化了一个多重PCR系统以同时扩增这些标记。该系统的准确性通过受控交配配对进行验证，随后应用于群体交配场景。（3）结果：所开发的多重PCR系统在受控和群体交配场景中都能高度准确地将后代与其亲本进行配对。（4）结论：该系统是小黄鱼谱系管理、选择性育种和保护工作的宝贵工具，有助于可持续水产养殖实践和遗传改良。
