Sun D Q, Li H Y, Xu T J, Wang R X
Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, PR China.
Genet Mol Res. 2012 May 21;11(2):1469-74. doi: 10.4238/2012.May.21.3.
The small yellow croaker (Larimichthys polyactis) is a highly valued fish for human consumption found in the Western Pacific that was considered endangered until recently because of overfishing. We selected microsatellite markers for this species from markers developed for Miichthys miiuy, also of the family Sciaenidae. Among 43 markers polymorphic for M. miiuy, 11 were found to be polymorphic for L. polyactis. Characterization of these 11 loci was made based on 30 L. polyactis individuals collected by trawling in the Zhoushan Fishing Ground, Zhejiang Province, China. Total genomic DNA was isolated from fin clips. The number of alleles per locus ranged from 4 to 10, with a mean of 5.82, while the effective number of alleles ranged from 1.64 to 10.00, with a mean of 3.22. Observed and expected heterozygosities ranged from 0.17 to 0.72 and from 0.39 to 0.81, respectively. Significant deviation from Hardy-Weinberg equilibrium was found at four loci, after applying Bonferroni's correction. There was no significant association between any of the pairs of microsatellite loci, hence allelic variation at these loci was considered independent. These 11 polymorphic microsatellite loci will be useful for genetic diversity analysis and molecular-assisted breeding for L. polyactis.
小黄鱼（Larimichthys polyactis）是一种在西太平洋发现的具有很高食用价值的鱼类，由于过度捕捞，直到最近它都被认为是濒危物种。我们从为同属鲷科的米氏鲷（Miichthys miiuy）开发的标记中为该物种选择了微卫星标记。在43个对米氏鲷具有多态性的标记中，发现有11个对小黄鱼具有多态性。基于在中国浙江省舟山渔场拖网采集的30个小黄鱼个体对这11个位点进行了特征分析。从鳍条中分离出总基因组DNA。每个位点的等位基因数范围为4至10，平均为5.82，而有效等位基因数范围为1.64至10.00，平均为3.22。观察到的杂合度和预期杂合度分别为0.17至0.72和0.39至0.81。在应用邦费罗尼校正后，发现有4个位点显著偏离哈迪-温伯格平衡。任何一对微卫星位点之间均无显著关联，因此这些位点的等位基因变异被认为是独立的。这11个多态性微卫星位点将有助于小黄鱼的遗传多样性分析和分子辅助育种。
