Wang Zheshu, Qu Liangjian, Fan Zhibin, Hou Luxuan, Hu Jianjun, Wang Lijuan
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China.
Biology (Basel). 2024 Sep 14;13(9):723. doi: 10.3390/biology13090723.
Poplar trees are significant for both economic and ecological purposes, and the fall webworm ( Drury) poses a major threat to their plantation in China. The preliminary resistance assessment in the previous research indicated that there were differences in resistance to the insect among these varieties, with '2KEN8' being more resistant and 'Nankang' being more susceptible. The present study analyzed the dynamic changes in the defensive enzymes and metabolic profiles of '2KEN8' and 'Nankang' at 24 hours post-infestation (hpi), 48 hpi, and 96 hpi. The results demonstrated that at the same time points, compared to susceptible 'Nankang', the leaf consumption by in '2KEN8' was smaller, and the larval weight gain was slower, exhibiting clear resistance to the insect. Biochemical analysis revealed that the increased activity of the defensive enzymes in '2KEN8' triggered by the feeding of was significantly higher than that of 'Nankang'. Metabolomics analysis indicated that '2KEN8' initiated an earlier and more intense reprogramming of the metabolic profile post-infestation. In the early stages of infestation, the differential metabolites induced in '2KEN8' primarily included phenolic compounds, flavonoids, and unsaturated fatty acids, which are related to the biosynthesis pathways of phenylpropanoids, flavonoids, unsaturated fatty acids, and jasmonates. The present study is helpful for identifying the metabolic biomarkers for inductive resistance to and lays a foundation for the further elucidation of the chemical resistance mechanism of poplar trees against this insect.
杨树在经济和生态方面都具有重要意义，而美国白蛾对中国杨树种植构成重大威胁。前期研究中的初步抗性评估表明，这些品种对该昆虫的抗性存在差异，‘2KEN8’抗性较强，‘南抗’较易感。本研究分析了‘2KEN8’和‘南抗’在接虫后24小时（hpi）、48 hpi和96 hpi时防御酶和代谢谱的动态变化。结果表明，在相同时间点，与易感的‘南抗’相比，‘2KEN8’的叶片被食叶量较小，幼虫体重增加较慢，对该昆虫表现出明显抗性。生化分析显示，美国白蛾取食引发的‘2KEN8’中防御酶活性增加显著高于‘南抗’。代谢组学分析表明，‘2KEN8’在接虫后启动了更早且更强烈的代谢谱重编程。在虫害早期阶段，‘2KEN8’中诱导产生的差异代谢物主要包括酚类化合物、黄酮类化合物和不饱和脂肪酸，它们与苯丙烷类、黄酮类、不饱和脂肪酸和茉莉酸类的生物合成途径有关。本研究有助于识别诱导抗美国白蛾的代谢生物标志物，并为进一步阐明杨树对该昆虫的化学抗性机制奠定基础。
