Jilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland, Jilin Agriculture University, Changchun, China.
College of Life Science, Jilin Agriculture University, Changchun, China.
Pestic Biochem Physiol. 2024 Jun;202:105969. doi: 10.1016/j.pestbp.2024.105969. Epub 2024 May 26.
Populus pseudo-cathayana × Populus deltoides is a crucial artificial forest tree species in Northeast China. The presence of the fall webworm (Hyphantria cunea) poses a significant threat to these poplar trees, causing substantial economic and ecological damage. This study conducted an insect-feeding experiment with fall webworm on P. pseudo-cathayana × P. deltoides, examining poplar's physiological indicators, transcriptome, and metabolome under different lengths of feeding times. Results revealed significant differences in phenylalanine ammonia-lyase activity, total phenolic content, and flavonoids at different feeding durations. Transcriptomic analysis identified numerous differentially expressed genes, including AP2/ERF, MYB, and WRKY transcription factor families exhibiting the highest expression variations. Differential metabolite analysis highlighted flavonoids and phenolic acid compounds of poplar's leaves as the most abundant in our insect-feeding experiment. Enrichment analysis revealed significant enrichment in the plant hormone signal transduction and flavonoid biosynthetic pathways. The contents of jasmonic acid and jasmonoyl-L-isoleucine increased with prolonged fall webworm feeding. Furthermore, the accumulation of dihydrokaempferol, catechin, kaempferol, and naringenin in the flavonoid biosynthesis pathway varied significantly among different samples, suggesting their crucial role in response to pest infestation. These findings provide novel insights into how poplar responds to fall webworm infestation.
毛白杨 × 加拿大杨是中国东北地区重要的人工林树种。美国白蛾对这些杨树构成了严重威胁，造成了巨大的经济和生态损失。本研究采用美国白蛾对毛白杨 × 加拿大杨进行取食实验，研究了不同取食时间对杨树生理指标、转录组和代谢组的影响。结果表明，不同取食时间下苯丙氨酸解氨酶活性、总酚含量和类黄酮含量存在显著差异。转录组分析鉴定出许多差异表达基因，包括 AP2/ERF、MYB 和 WRKY 转录因子家族，它们的表达变化最大。差异代谢物分析突出了杨树叶片中类黄酮和酚酸化合物的丰富度，在我们的昆虫取食实验中最为丰富。富集分析表明，植物激素信号转导和类黄酮生物合成途径显著富集。随着美国白蛾取食时间的延长，茉莉酸和茉莉酸-L-异亮氨酸的含量增加。此外，类黄酮生物合成途径中二氢山奈酚、儿茶素、山奈酚和柚皮素的积累在不同样品中差异显著，表明它们在应对虫害方面起着关键作用。这些发现为杨树对美国白蛾侵害的反应机制提供了新的见解。
