Department of Anesthesiology and Reanimation, Gazi University, Ankara 06560, Turkey.
Department of Physiology, Faculty of Medicine, Kutahya Health Sciences University, Kutahya 43020, Turkey.
Medicina (Kaunas). 2024 Sep 22;60(9):1552. doi: 10.3390/medicina60091552.
Sepsis and its related complications are associated with high morbidity and mortality, often leading to liver damage. Ozone, a molecule with anti-inflammatory and antioxidant properties, may offer protective effects. This study aimed to evaluate the therapeutic and protective impact of ozone on liver injury in a rat model of sepsis induced by cecal ligation and perforation (CLP). A total of 36 rats were randomly divided into five groups: control (Group C), ozone (Group O), cecal ligation and perforation (Group CLP), ozone + cecal ligation and perforation (Group O+CLP), and cecal ligation and perforation + ozone (Group CLP+O). In the ozone groups, 4 mL of ozone (20 µ/mL) was injected intraperitoneally. Biochemical and histopathological parameters were evaluated in liver tissue samples obtained at the end of 24 h. Polymorphonuclear leukocyte and monocyte infiltration and the total injury score were significantly reduced in the ozone-treated groups compared to the CLP group ( < 0.001). Tumor necrosis factor and interleukin 10 levels in the rat liver tissue were significantly reduced in the O+CLP and CLP+O groups compared to the CLP group, with the O+CLP group showing a more substantial decrease than the CLP+O group ( < 0.001). Thiobarbituric acid reactive substances and glutathione s-transferase levels were significantly lower in the ozone-treated groups compared to the CLP group ( < 0.001). Catalase activity was significantly elevated in the O+CLP group compared to the CLP group ( < 0.001). Serum aspartate transaminase, alanine transaminase, gamma-glutamyl transferase, and total bilirubin were significantly increased in the CLP group and decreased in the ozone-treated groups ( < 0.001, < 0.001, = 0.01, < 0.001 respectively). Administering ozone to rats one hour before the CLP significantly mitigated liver damage, showing a more pronounced effect compared to administering ozone one hour after CLP. The results indicate that ozone could serve a protective function in managing sepsis-induced liver damage.
脓毒症及其相关并发症与高发病率和死亡率相关,常导致肝损伤。臭氧作为一种具有抗炎和抗氧化特性的分子,可能具有保护作用。本研究旨在评估臭氧对盲肠结扎穿孔(CLP)诱导的脓毒症大鼠模型中肝损伤的治疗和保护作用。
将 36 只大鼠随机分为五组:对照组(C 组)、臭氧组(O 组)、盲肠结扎穿孔组(CLP 组)、臭氧+盲肠结扎穿孔组(O+CLP 组)和盲肠结扎穿孔+臭氧组(CLP+O 组)。在臭氧组中,经腹腔注射 4 mL 浓度为 20 µg/mL 的臭氧。24 h 后,取肝组织样本,评估生化和组织病理学参数。
与 CLP 组相比,臭氧处理组的多形核白细胞和单核细胞浸润以及总损伤评分明显降低(<0.001)。与 CLP 组相比,O+CLP 和 CLP+O 组大鼠肝组织中的肿瘤坏死因子和白细胞介素 10 水平明显降低,其中 O+CLP 组比 CLP+O 组降低更明显(<0.001)。与 CLP 组相比,臭氧处理组的硫代巴比妥酸反应物质和谷胱甘肽 S-转移酶水平明显降低(<0.001)。与 CLP 组相比,O+CLP 组的过氧化氢酶活性明显升高(<0.001)。CLP 组的血清天冬氨酸转氨酶、丙氨酸转氨酶、γ-谷氨酰转移酶和总胆红素明显升高,臭氧处理组则降低(<0.001,<0.001,=0.01,<0.001)。
在 CLP 前 1 小时给予大鼠臭氧可显著减轻肝损伤,与 CLP 后 1 小时给予臭氧相比,效果更明显。结果表明,臭氧在治疗脓毒症引起的肝损伤方面可能具有保护作用。