用于蚊子的喷雾干燥猪血粉的特性

Characteristics of a Spray-Dried Porcine Blood Meal for Mosquitoes.

作者信息

Weaver Alexander R, Rajagopal Nagarajan R, Pereira Roberto M, Koehler Philip G, MacIntosh Andrew J, Baldwin Rebecca W, Batich Christopher D

机构信息

Department of Chemical Engineering, University of Florida, 1030 Center Dr., Gainesville, FL 32611, USA.

Department of Materials Science and Engineering, University of Florida, 549 Gale Lemerand Drive, P.O. Box 11400, Gainesville, FL 32611, USA.

出版信息

Insects. 2024 Sep 19;15(9):716. doi: 10.3390/insects15090716.

DOI:10.3390/insects15090716

PMID:39336684

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11432713/

Abstract

Research into mosquito-borne illnesses faces hurdles because feeding fresh animal blood to rear female mosquitoes presents logistical, economic, and safety challenges. In this study, a shelf-stable additive (spray-dried porcine blood; SDPB) hypothesized to supply accessible hemoglobin was evaluated within an alternative meal (AM) containing whey powder and PBS for rearing the yellow fever mosquito . LC-MS/MS proteomics, microbial assays, and particle reduction techniques confirmed and characterized the functionality of hemoglobin in SDPB, while engorgement, fecundity, egg viability, and meal stability bioassays assessed AM performance. Chemical assays supported hemoglobin as the phagostimulant in SDPB with aggregates partially solubilized in the AM that can be more accessible via particle reduction. Unpaired two-tailed -tests indicate that the AM stimulates oogenesis (t = 13.6, = 0.003) and is stable under ambient (1+ y; t = 0.576, = 0.575) and aqueous (14 d; t = 0.515, = 0.639) conditions without decreasing fecundity. Egg hatch rates for the ninth generation of AM-reared were 50-70+%. With further development, this meal may serve as a platform for mass rearing or studying effects of nutritional additives on mosquito fitness due to its low cost and stability. Future work may examine tuning spray drying parameters and resulting impacts on hemoglobin agglomeration and feeding.

摘要

对蚊媒疾病的研究面临诸多障碍，因为给雌性蚊子喂食新鲜动物血液在后勤、经济和安全方面都存在挑战。在本研究中，一种被认为可提供可利用血红蛋白的稳定添加剂（喷雾干燥猪血；SDPB）在一种包含乳清粉和磷酸盐缓冲盐水（PBS）的替代饲料（AM）中进行了评估，用于饲养黄热病蚊子。液相色谱-串联质谱（LC-MS/MS）蛋白质组学、微生物检测和颗粒减少技术证实并表征了SDPB中血红蛋白的功能，而饱食、繁殖力、卵活力和饲料稳定性生物测定评估了AM的性能。化学分析支持血红蛋白是SDPB中的摄食刺激物，其聚集体在AM中部分溶解，通过颗粒减少可更易获取。非配对双尾t检验表明，AM刺激卵子发生（t = 13.6，P = 0.003），并且在环境（1年以上；t = 0.576，P = 0.575）和水性（14天；t = 0.515，P = 0.639）条件下稳定，不会降低繁殖力。用AM饲养的第九代蚊子的卵孵化率为50%-70%以上。随着进一步发展，这种饲料因其低成本和稳定性，可能成为大规模饲养或研究营养添加剂对蚊子健康影响的平台。未来的工作可能会研究调整喷雾干燥参数以及对血红蛋白团聚和摄食的影响。