Research Group in Environmental Factors of Neurodegenerative Diseases, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Red de Enfermedades Inflamatorias (REI), 28040 Madrid, Spain.
Department of Neurology, Hospital Universitario de Torrejón, 28850 Madrid, Spain.
Int J Mol Sci. 2024 Sep 17;25(18):10012. doi: 10.3390/ijms251810012.
microRNAs (miRNAs) are promising biomarkers for many diseases, including multiple sclerosis (MS). The neurofilament light chain (NfL) is a biomarker that can detect axonal damage in different neurological diseases. The objective of this study was to evaluate the association of the expression profile of pre-selected miRNAs and NfL levels with clinical and radiological variables in MS patients. We conducted a 1-year longitudinal prospective study in MS patients with different clinical forms. We measured clinical disability using the expanded disability status scale (EDSS), the magnetic resonance imaging (MRI) volumetry baseline, and cognitive functioning using the processing speed test (PST) at baseline and 1 year later. Selected serum miRNAs and serum NfL (sNfL) levels were quantified. Seventy-three patients were recruited. MiR-126.3p correlated with EDSS and cognitive status at baseline and miR-126.3p and miR-9p correlated with cognitive deterioration at 1 year. Correlations with regional brain volumes were observed between miR-126.3p and the cortical gray matter, cerebellum, putamen, and pallidum; miR-146a.5p with the cerebellum and pallidum; miR-29b.3p with white matter and the pallidum; miR-138.5p with the pallidum; and miR-9.5p with the thalamus. sNfL was correlated with miR-9.5p. miR-146a.5p was also associated with the MS phenotype. These data justify future studies to further explore the utility of miRNAs (mirR-126.3p, miR-146.5p, and miR.9-5p) and sNfL levels as biomarkers of MS.
microRNAs (miRNAs) 是许多疾病（包括多发性硬化症）有前途的生物标志物。神经丝轻链（NfL）是一种生物标志物，可以检测不同神经疾病中的轴突损伤。本研究的目的是评估预筛选 miRNA 的表达谱和 NfL 水平与 MS 患者的临床和影像学变量之间的关联。我们对具有不同临床形式的 MS 患者进行了为期 1 年的前瞻性纵向研究。我们使用扩展残疾状况量表（EDSS）评估临床残疾，使用基线磁共振成像（MRI）容积测量和认知功能处理速度测试（PST）在基线和 1 年后进行评估。测量了选定的血清 miRNA 和血清 NfL（sNfL）水平。共招募了 73 名患者。miR-126.3p 与基线时的 EDSS 和认知状态相关，miR-126.3p 和 miR-9p 与 1 年后的认知恶化相关。miR-126.3p 与皮质灰质、小脑、壳核和苍白球；miR-146a.5p 与小脑和苍白球；miR-29b.3p 与白质和苍白球；miR-138.5p 与苍白球；miR-9.5p 与丘脑之间观察到与区域脑体积的相关性。sNfL 与 miR-9.5p 相关。miR-146a.5p 也与 MS 表型相关。这些数据证明了进一步探索 miRNA（mirR-126.3p、miR-146.5p 和 miR.9-5p）和 sNfL 水平作为 MS 生物标志物的效用的未来研究是合理的。
