Department of Medical Microbiology, Faculty of Medicine, Medical University of Gdansk, Debowa 25, 80-204 Gdansk, Poland.
Department of Physiopathology, Medical University of Gdansk, Debinki 7, 80-211 Gdansk, Poland.
Int J Mol Sci. 2024 Sep 18;25(18):10050. doi: 10.3390/ijms251810050.
Pandemics from viral respiratory tract infections in the 20th and early 21st centuries were associated with high mortality, which was not always caused by a primary viral infection. It has been observed that severe course of infection, complications and mortality were often the result of co-infection with other pathogens, especially . During the COVID-19 pandemic, it was also noticed that patients infected with had a significantly higher mortality rate (61.7%) compared to patients infected with SARS-CoV-2 alone. Our previous studies have shown that strains isolated from patients with COVID-19 had a different protein profile than the strains in non-COVID-19 patients. Therefore, this study aims to analyze strains isolated from COVID-19 patients in terms of their pathogenicity by analyzing their virulence genes, adhesion, cytotoxicity and penetration to the human pulmonary epithelial cell line A549. We have observed that half of the tested strains isolated from patients with COVID-19 had a necrotizing effect on the A549 cells. The strains also showed greater variability in terms of their adhesion to the human cells than their non-COVID-19 counterparts.
20 世纪和 21 世纪初的病毒性呼吸道感染大流行与高死亡率相关，而这些死亡率并不总是由原发性病毒感染引起的。人们观察到，感染的严重程度、并发症和死亡率往往是其他病原体合并感染的结果，特别是。在 COVID-19 大流行期间，人们还注意到，感染 的患者的死亡率（61.7%）明显高于单独感染 SARS-CoV-2 的患者。我们之前的研究表明，从 COVID-19 患者中分离出的 菌株与非 COVID-19 患者中的菌株相比，具有不同的蛋白谱。因此，本研究旨在通过分析其毒力基因、黏附性、细胞毒性和对人肺上皮细胞系 A549 的侵袭性，分析从 COVID-19 患者中分离出的 菌株的致病性。我们观察到，从 COVID-19 患者中分离出的一半 菌株对 A549 细胞具有坏死作用。这些菌株在黏附到人类细胞方面的变异性也明显大于其非 COVID-19 对应物。
