Department of Medical Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdańsk, Poland.
Chair of Microbiology, Immunology and Laboratory Medicine, Department of Laboratory Medicine, Pomeranian Medical University in Szczecin, Szczecin, Poland.
Microb Drug Resist. 2021 Jul;27(7):956-964. doi: 10.1089/mdr.2020.0399. Epub 2021 Mar 3.
The biofilm-forming strains are responsible for causing a number of diseases. With the emergence of multidrug resistance they constitute a catastrophic threat to medicine. The ability of 65 clinical strains of multidrug-resistant (MDRSA) to form biofilm was examined in this study and analyzed in relation to SCC, type, microbial surface components recognizing adhesive matrix molecules (MSCRAMMs), and genes. Results obtained from crystal violet and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assays showed that all MDRSA strains tested form biofilm but, of 65 strains, only 18 strains (28%) were found to form a biofilm with high metabolic activity and a great amount of biomass. The high proportion of MDRSA isolates in our study made no significant difference for and MSCRAMMs genes according to biofilm-forming capacity, except for , , and gene. In addition, this study demonstrated that strains carrying SCC type I showed a significantly decreased biofilm viability compared with the strains harboring SCC type II and type IV, but SCC type could not serve as a good predictor of biofilm formation. However, we found that significantly weaker metabolic activity was detected in the biofilm of isolates with type t011.
生物膜形成菌株是引起许多疾病的原因。随着多药耐药性的出现，它们对医学构成了灾难性的威胁。本研究检测了 65 株临床多药耐药性金黄色葡萄球菌 (MDRSA) 形成生物膜的能力，并分析了它们与 SCC、类型、微生物表面成分识别黏附基质分子 (MSCRAMMs) 和基因的关系。结晶紫和 MTT [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐] 测定结果表明，所有测试的 MDRSA 菌株都能形成生物膜，但在 65 株菌株中，只有 18 株 (28%) 形成具有高代谢活性和大量生物量的生物膜。我们的研究中，MDRSA 分离株的高比例在生物膜形成能力方面，除了 、 、和 基因外，与 和 MSCRAMMs 基因没有显著差异。此外，本研究表明，携带 SCC 类型 I 的菌株与携带 SCC 类型 II 和 IV 的菌株相比，生物膜活力显著降低，但 SCC 类型不能作为生物膜形成的良好预测因子。然而，我们发现，具有 类型 t011 的分离株的生物膜中代谢活性明显较弱。
