Fowora Muinah Adenike, Aiyedogbon Adenike, Omolopo Ibilola, Tajudeen Ahmed Oluwasegun, Onyeaghasiri Faustina, Edu-Muyideen Idowu, Olanlege Abdul-Lateef Olatunde, Abioye Aminah, Bamidele Tajudeen Akanji, Raheem Toyosi, Adesesan Adesegun, Iwalokun Bamidele, Salako Babatunde Lawal
Department of Molecular Biology and Biotechnology, Nigerian Institute of Medical Research, Lagos, Nigeria.
FOWM Biotechnology Laboratories, Lagos, Nigeria.
Mol Biol Rep. 2024 May 22;51(1):665. doi: 10.1007/s11033-024-09578-3.
Staphylococcus aureus (S. aureus) associated with COVID-19 has not been well documented. This cross-sectional study evaluated the association between nasal S. aureus carriage and COVID-19.
Nasopharyngeal samples were collected from 391 participants presenting for COVID-19 test in Lagos, Nigeria, and S. aureus was isolated from the samples. Antimicrobial susceptibility test was done by disc diffusion method. All S. aureus isolates were screened for the presence of mecA, panton-valentine leucocidin (PVL) and toxic shock syndrome toxin (TSST) virulence genes by polymerase chain reaction. Staphylococcal protein A (spa) typing was conducted for all the isolates. Participants with COVID-19 had double the prevalence of S. aureus (42.86%) compared to those who tested negative (20.54%). A significant association was seen between S. aureus nasal carriage and COVID-19 (p = 0.004). Antimicrobial sensitivity results showed resistance to oxacillin (100%), cefoxitin (53%), and vancomycin (98.7%). However, only 41% of the isolates harbored the mecA gene, with SCCmecV being the most common SCCmec type. There was no association between the carriage of virulence genes and COVID-19. A total of 23 Spa types were detected, with t13249 and t095 being the two most common spa types.
This study examined the association between nasal S. aureus carriage and SARS-COV-2 infection. Further research is required to fully explore the implications of S. aureus co-infection with COVID-19.
与新型冠状病毒肺炎（COVID-19）相关的金黄色葡萄球菌（金葡菌）尚未得到充分记录。这项横断面研究评估了鼻腔金葡菌携带与COVID-19之间的关联。
从尼日利亚拉各斯391名前来进行COVID-19检测的参与者中采集鼻咽样本，并从样本中分离出金葡菌。采用纸片扩散法进行药敏试验。通过聚合酶链反应对所有金葡菌分离株进行mecA、杀白细胞素（PVL）和中毒性休克综合征毒素（TSST）毒力基因检测。对所有分离株进行葡萄球菌蛋白A（spa）分型。COVID-19患者金葡菌携带率（42.86%）是检测阴性者（20.54%）的两倍。金葡菌鼻腔携带与COVID-19之间存在显著关联（p = 0.004）。药敏结果显示对苯唑西林（100%）、头孢西丁（53%）和万古霉素（98.7%）耐药。然而，只有41%的分离株携带mecA基因，SCCmecV是最常见的SCCmec类型。毒力基因携带与COVID-19之间无关联。共检测到23种spa型，t13249和t095是最常见的两种spa型。
本研究探讨了鼻腔金葡菌携带与严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染之间的关联。需要进一步研究以充分探索金葡菌与COVID-19合并感染的影响。
