May S R, Wainwright J F, DeClement F A
Burns Incl Therm Inj. 1985 Oct;12(1):36-48. doi: 10.1016/0305-4179(85)90181-0.
The availability of cryopreservation and low temperature storage techniques for cadaveric allograft skin allows it to be preserved while microbial assessments are made before its use as a temporary biological dressing on burn wounds. In a 300-donor, 5-year prospective study, we tested ten skin samples from defined areas on each donor for microbiological contamination. Although the skin from 52.3 per cent of the donors possessed some detectable residual microbial contamination after surgical body preparation and skin removal, such contamination was limited to an average of 1.4 areas per body, leaving 86 per cent of all skin obtained free from detectable contamination and suitable for use as biological wound dressings. The number of skin samples tested per donor body determined the accuracy of detection of the presence of contamination. Testing one skin sample per donor body yielded a correct skin assessment 92 per cent of the time, while testing five skin samples increased the accuracy to 96 per cent, and testing ten skin samples yielded a 99.9 per cent accuracy in detection of skin contamination. Thus, it is within the ability of a skin bank to set the limits of microbiological risk to patients receiving processed cadaveric allograft skin.
尸体同种异体皮肤的冷冻保存和低温储存技术，使得在将其用作烧伤创面的临时生物敷料之前进行微生物评估时，它能够被保存下来。在一项对300名供体进行的为期5年的前瞻性研究中，我们对每个供体特定部位的10个皮肤样本进行了微生物污染检测。尽管在手术备皮和取皮后，52.3%的供体皮肤存在一些可检测到的残余微生物污染，但这种污染平均每个供体仅限于1.4个部位，所有获取的皮肤中有86%没有可检测到的污染，适合用作生物伤口敷料。每个供体检测的皮肤样本数量决定了污染存在检测的准确性。每个供体检测一个皮肤样本时，皮肤评估正确的时间占92%，检测五个皮肤样本时准确性提高到96%，检测十个皮肤样本时皮肤污染检测的准确性达到99.9%。因此，皮肤库有能力设定接受处理过的尸体同种异体皮肤的患者所面临的微生物风险限度。
