Rácz Richárd, Gellért Ákos, Papp Tibor, Doszpoly Andor
HUN-REN Veterinary Medical Research Institute, H-1143 Budapest, Hungary.
Life (Basel). 2024 Aug 23;14(9):1050. doi: 10.3390/life14091050.
The effectiveness of acyclovir, a selective anti-herpesvirus agent, was tested both in silico and in vitro against two ranaviruses, namely the European catfish virus (ECV) and Frog virus 3 (FV3). ECV can cause significant losses in catfish aquaculture, while FV3 poses a risk to vulnerable amphibian populations. The genome of ranaviruses encodes thymidine kinases (TKs) similar to those of herpesviruses. Molecular docking simulations demonstrated that the acyclovir molecule can bind to the active sites of both investigated viral TKs in an orientation conducive to phosphorylation. Subsequently, the antiviral effect of acyclovir was tested in vitro in Epithelioma Papulosum Cyprini (EPC) cells with endpoint titration and qPCR. Acyclovir was used at a concentration of 800 µM, which significantly reduced the viral loads and titers of the ranaviruses. A similar reduction rate was observed with Ictalurid herpesvirus 2, which was used as a positive control virus. These promising results indicate that acyclovir might have a wider range of uses; besides its effectiveness against herpesviruses, it could also be used against ranavirus infections.
阿昔洛韦是一种选择性抗疱疹病毒药物，其有效性在计算机模拟和体外实验中针对两种蛙病毒进行了测试，这两种蛙病毒分别是欧洲鲶鱼病毒（ECV）和蛙病毒3型（FV3）。ECV可在鲶鱼养殖中造成重大损失，而FV3对易受影响的两栖动物种群构成风险。蛙病毒的基因组编码与疱疹病毒相似的胸苷激酶（TKs）。分子对接模拟表明，阿昔洛韦分子能够以有利于磷酸化的方向结合到两种被研究病毒TKs的活性位点上。随后，通过终点滴定和qPCR在鲤上皮瘤（EPC）细胞中对阿昔洛韦的抗病毒效果进行了体外测试。阿昔洛韦的使用浓度为800µM，这显著降低了蛙病毒的病毒载量和滴度。作为阳性对照病毒的斑点叉尾鮰疱疹病毒2也观察到了类似的降低率。这些有前景的结果表明，阿昔洛韦可能具有更广泛的用途；除了对疱疹病毒有效外，它还可用于对抗蛙病毒感染。
