de Souza Paula Araujo, Santos Milena Cristina Silva Dos, de Miranda Rebeca Vitória da Silva Lage, da Costa Luciana Veloso, da Silva Raphael Paiva Paschoal, de Miranda Catia Aparecida Chaia, da Silva Ana Paula Roque, Forsythe Stephen James, Bôas Maria Helena Simões Villas, Brandão Marcelo Luiz Lima
Laboratory of Microbiology of Food and Sanitizes, INCQS/Fiocruz, Rio de Janeiro 21040-900, Brazil.
Laboratory of Microbiological Control, Bio-Manguinhos/Fiocruz, Rio de Janeiro 21040-900, Brazil.
Life (Basel). 2024 Aug 29;14(9):1079. doi: 10.3390/life14091079.
This study aimed to characterize strains isolated from hospitalized patients during the COVID-19 pandemic. This was achieved using phenotypic and molecular techniques, including their antimicrobial resistance profile and biofilm formation. Eighteen strains were isolated from a hospital in Rio de Janeiro, Brazil, and identified by VITEK2, MALDI-TOF/MS (VITEK MS and MALDI Biotyper), and 16S rRNA sequencing. Fourier-transform infrared (FTIR) spectroscopy, antimicrobial susceptibility testing, and biofilm formation and disinfectant tolerance tests were applied to evaluate the virulence characteristics of the strains. VITEK2 (≥99%), VITEK MS (≥82.7%), and MALDI Biotyper (score ≥ 2.01) accurately identified the strains, but 16S rRNA sequencing did not differentiate the species from . FTIR typing identified three different clusters, but no correlation between the phenotypical or antimicrobial susceptibility testing patterns was found. Most strains exhibited resistance to various antimicrobials. The exceptions were sensitivity to amikacin and norfloxacin, and consequently, these could be considered potential treatment options. Most strains (n = 15, 83.3%) produced biofilms on polystyrene. Sodium hypochlorite treatment (0.5%/15 min) was shown to be the most effective disinfectant for biofilm elimination. biofilm formation and tolerance to disinfectants demonstrate the need for effective cleaning protocols to eliminate contamination by this organism in the hospital environment and medical equipment.
本研究旨在对新冠疫情期间从住院患者中分离出的菌株进行特征描述。这是通过表型和分子技术实现的，包括它们的抗菌药物耐药谱和生物膜形成情况。从巴西里约热内卢的一家医院分离出18株菌株，并通过VITEK2、基质辅助激光解吸电离飞行时间质谱（VITEK MS和MALDI Biotyper）以及16S rRNA测序进行鉴定。应用傅里叶变换红外（FTIR）光谱、抗菌药物敏感性测试、生物膜形成和消毒剂耐受性测试来评估这些菌株的毒力特征。VITEK2（≥99%）、VITEK MS（≥82.7%）和MALDI Biotyper（评分≥2.01）准确鉴定了这些菌株，但16S rRNA测序未能区分[具体物种1]与[具体物种2]。FTIR分型确定了三个不同的聚类，但未发现表型或抗菌药物敏感性测试模式之间的相关性。大多数菌株对各种抗菌药物表现出耐药性。例外的是对阿米卡星和诺氟沙星敏感，因此，这些可被视为潜在的治疗选择。大多数菌株（n = 15，83.3%）在聚苯乙烯上形成生物膜。结果表明，次氯酸钠处理（0.5%/15分钟）是消除生物膜最有效的消毒剂。生物膜形成和对消毒剂的耐受性表明，需要有效的清洁方案来消除医院环境和医疗设备中该生物体的污染。
