Bauss F, Minne H W, Sterz H, Weng U, Wesch H, Ziegler R
Calcif Tissue Int. 1985 Sep;37(5):539-46. doi: 10.1007/BF02557838.
Various methods exist for determination of trabecular or total bone mass in animal experiments. There is one group of simple techniques not requiring sophisticated equipment focusing on bone-calcium determination. By contrast, another group of newer methods requires complex equipment for procedures such as computerized analysis of bone X-rays or of nondecalcified bone sections. The methods of the first group require considerable time to perform, whereas those of the second group allow a great number of analyses in a short time. We have adapted the computerized techniques to the determination of rat-bone mass and then compared both types of methodology using the new animal model for pathological loss of bone mass: the syndrome of inflammation-mediated osteopenia (IMO) in rats [1, 2]. Reliable results were obtained with both approaches, but we recommend the use of one of the new techniques in cases where a large number of analyses is required.
在动物实验中，存在多种测定小梁骨或总骨量的方法。有一组简单技术，不需要复杂设备，重点在于骨钙测定。相比之下，另一组较新的方法需要复杂设备来进行诸如骨X射线或未脱钙骨切片的计算机分析等程序。第一组方法执行起来需要相当长的时间，而第二组方法则可以在短时间内进行大量分析。我们已将计算机技术应用于大鼠骨量的测定，然后使用新的骨量病理性丢失动物模型：大鼠炎症介导的骨质减少综合征（IMO）[1,2]，对这两种方法进行了比较。两种方法都获得了可靠的结果，但我们建议在需要进行大量分析的情况下使用其中一种新技术。
