Gopalakrishnan Vinoj, Saxena Priya, Thakur Payal, Lipatov Alexey, Sani Rajesh K
Department of Chemical and Biological Engineering, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA.
Data Driven Material Discovery Center for Bioengineering Innovation, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA.
Microorganisms. 2024 Aug 24;12(9):1759. doi: 10.3390/microorganisms12091759.
Bacterial adhesion and biofilm maturation is significantly influenced by surface properties, encompassing both bare surfaces and single or multi-layered coatings. Hence, there is an utmost interest in exploring the intricacies of gene regulation in sulfate-reducing bacteria (SRB) on copper and graphene-coated copper surfaces. In this study, G20 was used as the model SRB to elucidate the pathways that govern pivotal roles during biofilm formation on the graphene layers. Employing a potent reporter green fluorescent protein (GFP) tagged to G20, the spatial structure of G20 biofilm on copper foil (CuF), single-layer graphene-coated copper (Cu-GrI), and double-layer graphene-coated copper (Cu-GrII) surfaces was investigated. Biofilm formation on CuF, Cu-GrI, and Cu-GrII surfaces was quantified using CLSM z-stack images within COMSTAT v2 software. The results revealed that CuF, Cu-GrI, and Cu-GrII did not affect the formation of the GFP-tagged O. G20 biofilm architecture. qPCR expression showed insignificant fold changes for outer membrane components regulating the quorum-sensing system, and global regulatory proteins between the uncoated and coated surfaces. Notably, a significant expression was observed within the sulfate reduction pathway confined to dissimilatory sulfite reductases on the Cu-GrII surface compared to the CuF and Cu-GrI surfaces.
细菌的黏附以及生物膜成熟受到表面特性的显著影响，这些表面特性包括裸露表面以及单层或多层涂层。因此，人们对探究硫酸盐还原菌（SRB）在铜表面和石墨烯涂层铜表面的基因调控复杂性有着极大的兴趣。在本研究中，G20被用作模型SRB，以阐明在石墨烯层上生物膜形成过程中起关键作用的调控途径。利用标记有强力报告基因绿色荧光蛋白（GFP）的G20，研究了G20生物膜在铜箔（CuF）、单层石墨烯涂层铜（Cu-GrI）和双层石墨烯涂层铜（Cu-GrII）表面的空间结构。使用COMSTAT v2软件中的CLSM z-stack图像对CuF、Cu-GrI和Cu-GrII表面的生物膜形成进行了定量分析。结果表明，CuF、Cu-GrI和Cu-GrII对标记有GFP的O. G20生物膜结构的形成没有影响。qPCR表达显示，在调控群体感应系统的外膜成分以及未涂层和涂层表面之间的全局调控蛋白方面，折叠变化不显著。值得注意的是，与CuF和Cu-GrI表面相比，在Cu-GrII表面的异化亚硫酸盐还原酶所在的硫酸盐还原途径中观察到显著表达。
