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主要穹窿蛋白/肺耐药相关蛋白：一种用于炎症和急性感染的新型生物标志物。

Major Vault Protein/Lung Resistance-Related Protein: A Novel Biomarker for Inflammation and Acute Infections.

作者信息

Routsias John G, Marinou Dionysia, Mavrouli Maria, Tsakris Athanasios, Pitiriga Vassiliki C

机构信息

Department of Microbiology, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece.

出版信息

Microorganisms. 2024 Aug 25;12(9):1762. doi: 10.3390/microorganisms12091762.

DOI:10.3390/microorganisms12091762
PMID:39338437
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11434279/
Abstract

INTRODUCTION

Vault particles are large cytoplasmic ribonucleoprotein particles that participate in inflammation. The aim of this study was to assess the diagnostic and prognostic value of major vault protein (MVP) in patients with inflammation, in order to determine whether MVP could be used as a biomarker for infection or inflammation. We also aimed to compare the diagnostic impact of MVP compared to other conventional measurements, such as CRP or white blood cell (WBC) counts.

METHODS

CRP and MVP levels were measured in 111 sera samples from 85 patients with inflammation admitted to a tertiary-care hospital and 26 healthy individuals during an 18-month period (2019-2020), using nephelometry and a custom MVP sandwich ELISA assay, respectively. In addition, WBC counts were measured using a commercial assay.

RESULTS

MVP levels were found to be elevated in patients with inflammation compared to healthy individuals ( < 0.0001). Moreover, MVP levels were higher in patients with inflammation due to an infectious etiology compared to those with non-infectious etiology ( = 0.0006). MVP levels significantly decreased during the first four days of infection in response to antibiotic treatment, while CRP levels showed a less-sensitive decline. An ROC curve analysis demonstrated that MVP and CRP have similarly high diagnostic accuracy, with AUCs of 0.955 and 0.995, respectively, followed by WBCs with an AUC of 0.805.

CONCLUSIONS

The ROC curves demonstrated that MVP has the potential to serve as a diagnostic biomarker for inflammation and infection. Additionally, MVP levels may reflect the efficacy of antibiotic treatment.

摘要

引言

穹窿体颗粒是参与炎症反应的大型细胞质核糖核蛋白颗粒。本研究旨在评估主要穹窿体蛋白（MVP）在炎症患者中的诊断和预后价值，以确定MVP是否可作为感染或炎症的生物标志物。我们还旨在比较MVP与其他传统检测指标（如C反应蛋白（CRP）或白细胞（WBC）计数）的诊断影响。

方法

在18个月期间（2019 - 2020年），分别使用散射比浊法和定制的MVP夹心酶联免疫吸附测定法，对一家三级医院收治的85例炎症患者的111份血清样本以及26名健康个体的血清样本进行CRP和MVP水平检测。此外，使用商业检测方法测量WBC计数。

结果

与健康个体相比，炎症患者的MVP水平升高（<0.0001）。此外，与非感染性病因引起的炎症患者相比，感染性病因引起的炎症患者的MVP水平更高（=0.0006）。在感染的前四天，MVP水平因抗生素治疗而显著下降，而CRP水平下降的敏感性较低。ROC曲线分析表明，MVP和CRP具有相似的高诊断准确性，AUC分别为0.955和0.995，其次是WBC，AUC为0.805。

结论

ROC曲线表明MVP有潜力作为炎症和感染的诊断生物标志物。此外，MVP水平可能反映抗生素治疗的疗效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/2062b389f566/microorganisms-12-01762-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/fc79fdf3a1c7/microorganisms-12-01762-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/ee91eb2b98ea/microorganisms-12-01762-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/03ffdad58971/microorganisms-12-01762-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/fa5c2a598306/microorganisms-12-01762-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/fff18c895d42/microorganisms-12-01762-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/6d50a7708986/microorganisms-12-01762-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/b8a358e0d550/microorganisms-12-01762-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/029d2f386d31/microorganisms-12-01762-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/046f384d1282/microorganisms-12-01762-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/2062b389f566/microorganisms-12-01762-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/fc79fdf3a1c7/microorganisms-12-01762-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/ee91eb2b98ea/microorganisms-12-01762-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/03ffdad58971/microorganisms-12-01762-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/fa5c2a598306/microorganisms-12-01762-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/fff18c895d42/microorganisms-12-01762-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/6d50a7708986/microorganisms-12-01762-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/b8a358e0d550/microorganisms-12-01762-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/029d2f386d31/microorganisms-12-01762-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/046f384d1282/microorganisms-12-01762-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1391/11434279/2062b389f566/microorganisms-12-01762-g010.jpg

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本文引用的文献

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Senescent Macrophages Release Inflammatory Cytokines and RNA-Loaded Extracellular Vesicles to Circumvent Fibroblast Senescence.衰老的巨噬细胞释放炎性细胞因子和载有RNA的细胞外囊泡以规避成纤维细胞衰老。
Biomedicines. 2024 May 14;12(5):1089. doi: 10.3390/biomedicines12051089.
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Major vault protein/lung resistance related protein: a novel biomarker for rheumatoid arthritis.主要穹窿蛋白/肺耐药相关蛋白：类风湿关节炎的一种新型生物标志物。
Clin Exp Rheumatol. 2021 Sep-Oct;39(5):1033-1042. doi: 10.55563/clinexprheumatol/pcozc1. Epub 2020 Oct 29.
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Major vault protein suppresses obesity and atherosclerosis through inhibiting IKK-NF-κB signaling mediated inflammation.
主要穹窿蛋白通过抑制 IKK-NF-κB 信号转导介导的炎症来抑制肥胖和动脉粥样硬化。
Nat Commun. 2019 Apr 17;10(1):1801. doi: 10.1038/s41467-019-09588-x.
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Inducible Major Vault Protein Plays a Pivotal Role in Double-Stranded RNA- or Virus-Induced Proinflammatory Response.可诱导的主要穹窿蛋白在双链RNA或病毒诱导的促炎反应中起关键作用。
J Immunol. 2016 Mar 15;196(6):2753-66. doi: 10.4049/jimmunol.1501481. Epub 2016 Feb 3.
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Human hepatitis B virus surface and e antigens inhibit major vault protein signaling in interferon induction pathways.人乙型肝炎病毒表面和 e 抗原抑制干扰素诱导途径中的主要穹窿蛋白信号传导。
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Structural studies of large nucleoprotein particles, vaults.大核蛋白颗粒、穹顶的结构研究。
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