Patić Aleksandra, Kovačević Gordana, Vuković Vladimir, Hrnjaković Cvjetković Ivana, Ristić Mioljub, Milosavljević Biljana, Medić Deana, Djilas Milan, Radovanov Jelena, Kovačević Aleksandra, Pustahija Tatjana, Balać Dragana, Petrović Vladimir
Institute of Public Health of Vojvodina, 21000 Novi Sad, Serbia.
Department of Microbiology with Parasitology and Immunology, Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia.
Microorganisms. 2024 Aug 30;12(9):1800. doi: 10.3390/microorganisms12091800.
The currently dominant SARS-CoV-2 omicron variant, while causing mild respiratory symptoms, exhibits high transmissibility, drug resistance, and immune evasion. We investigated whether the presence of the SARS-CoV-2 affected the dynamics of fecal microbial composition isolated in culture in moderate COVID-19 patients. Blood, stool, and medical records were collected from 50 patients with confirmed SARS-CoV-2 infection. Two samples were taken per patient, at disease onset (within 5 days) and after symptom resolution (30-35 days). The part of the gut microbiota identifiable using MALDI-TOF MS was analyzed, and inflammatory cytokines and blood markers were measured in serum. The analysis identified 566 isolates at the species level, including 83 bacterial and 9 fungal species. Our findings indicate a change in the gut microbiota composition isolated in culture during the initial phase of infection, characterized by the proliferation of opportunistic bacteria such as spp. and spp., at the expense of beneficial commensal bacteria from the genus and . Additionally, the enrichment of fungal pathogens in fecal samples collected 30 days after the cessation of disease symptoms might suggest a prolonged disruption of the gut microbiota even after the resolution of COVID-19 symptoms. This study contributes to a growing body of evidence on the systemic effects of SARS-CoV-2 and highlights the importance of considering gastrointestinal involvement in the management and treatment of COVID-19.
目前占主导地位的新冠病毒奥密克戎变体虽然引起的呼吸道症状较轻，但具有高传播性、耐药性和免疫逃逸能力。我们调查了新冠病毒的存在是否会影响中度新冠肺炎患者培养分离出的粪便微生物组成动态。从50例确诊感染新冠病毒的患者中收集血液、粪便和病历。每位患者在疾病发作时（5天内）和症状缓解后（30 - 35天）采集两份样本。分析了使用基质辅助激光解吸电离飞行时间质谱（MALDI - TOF MS）可识别的肠道微生物群部分，并检测了血清中的炎性细胞因子和血液标志物。该分析在物种水平上鉴定出566株分离株，包括83种细菌和9种真菌。我们的研究结果表明，在感染初期，培养分离出的肠道微生物群组成发生了变化，其特征是机会性细菌如 spp. 和 spp. 的增殖，而以来自 属和 属的有益共生细菌为代价。此外，疾病症状停止30天后收集的粪便样本中真菌病原体的富集可能表明，即使新冠肺炎症状缓解后，肠道微生物群仍受到长期破坏。这项研究为越来越多关于新冠病毒全身影响的证据做出了贡献，并强调了在新冠肺炎管理和治疗中考虑胃肠道受累情况的重要性。
