Zhu Xiaowen, Zhou Zhiyu, Cheng Yang, Deng Ziqi, Wu Hao, Nussio Luiz Gustavo, Zhou Zhenming, Meng Qingxiang
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Forage Quality and Conservation Lab, Department of Animal Sciences, Luiz de Queiroz College of Agriculture, University of Sao Paulo, Sao Paulo 05508-220, Brazil.
Microorganisms. 2024 Sep 3;12(9):1825. doi: 10.3390/microorganisms12091825.
The aim of this experiment is to explore the effect of sodium sulfate (NaSO) on methane reduction in the rumen, and its impact on anaerobic methane-oxidizing archaea (ANME). Using mixed rumen fluid from four Angus cattle fistulas, this study conducted an fermentation. Adding NaSO to the fermentation substrate resulted in sulfur concentrations in the substrate of 0.4%, 0.6%, 0.8%, 1.0%, 1.2%, 1.4%, 1.6%, 1.8%, 2.0%, 2.2%, and 2.4%. The gas production rate and methane yield were measured using an gas production method. Subsequently, the fermentation fluid was collected to determine the fermentation parameters. The presence of ANME in the fermentation broth, as well as the relationship between the number of bacteria, archaea, sulfate reducing bacteria (SRB), ANME, and the amount of NaSO added to the substrate, were measured using qPCR. The results showed that: (1) the addition of NaSO could significantly reduce CH production and was negatively correlated with CO production; (2) ANME-1 and ANME-2c did exist in the fermentation broth; (3) the total number of archaea, SRB, ANME-1, and ANME-2c increased with the elevation of NaSO. The above results indicated that NaSO could mitigate methane production via sulfate-dependent anaerobic methane oxidation (S-DAMO) in the rumen. In the future management of beef cattle, including sodium sulfate in their diet can stimulate S-DAMO activity, thereby promoting a reduction in methane emissions.
本实验的目的是探究硫酸钠（NaSO）对瘤胃中甲烷减排的影响及其对厌氧甲烷氧化古菌（ANME）的影响。本研究使用来自四头安格斯牛瘘管的混合瘤胃液进行发酵。向发酵底物中添加NaSO后，底物中的硫浓度分别为0.4%、0.6%、0.8%、1.0%、1.2%、1.4%、1.6%、1.8%、2.0%、2.2%和2.4%。采用产气法测定产气速率和甲烷产量。随后，收集发酵液以确定发酵参数。使用qPCR测定发酵液中ANME的存在情况，以及细菌、古菌、硫酸盐还原菌（SRB）、ANME的数量与添加到底物中的NaSO量之间的关系。结果表明：（1）添加NaSO可显著降低CH产量，且与CO产量呈负相关；（2）发酵液中确实存在ANME-1和ANME-2c；（3）古菌、SRB、ANME-1和ANME-2c的总数随NaSO浓度的升高而增加。上述结果表明，NaSO可通过瘤胃中依赖硫酸盐的厌氧甲烷氧化（S-DAMO）减轻甲烷生成。在未来肉牛的管理中，在其日粮中添加硫酸钠可刺激S-DAMO活性，从而促进甲烷排放的减少。
