Martins Jéssica Letícia Abreu, Franzin Mayara Loss, Ferreira Douglas da Silva, Magina Larissa Cristina Rocha, Martins Elem Fialho, Mendonça Laís Viana Paes, Neves Wânia Dos Santos, Pallini Angelo, Valicente Fernando Hercos, Schmidt Jason M, Elliot Simon Luke, Venzon Madelaine
Department of Entomology, Federal University of Viçosa, Viçosa 36570-900, Brazil.
Nestlé Enterprise, São Paulo 04730-090, Brazil.
Microorganisms. 2024 Sep 6;12(9):1845. doi: 10.3390/microorganisms12091845.
(Hypocreales: Clavicipitaceae) has a multifunctional life cycle, establishing as a plant endophyte and acting as entomopathogenic fungi. and can be associated with coffee plants and provide enhanced protection against a major pest of coffee, the coffee leaf miner (CLM) (). This association would be an easily deployable biological control option. Here we tested the potential of inoculating coffee seeds with and collected from the soil of coffee crops in the Cerrado (Brazil) for control of the CLM and the enhancement of plant growth with a commonly used fungicide. We conducted the experiment in a greenhouse and after the seedlings grew, we placed them in a cage with two couples of CLMs. We evaluated the CLM development time, reproduction, and plant growth traits. We observed a longer development time of CLMs when fed on plants inoculated with both isolates. In addition, the CLMs laid fewer eggs compared to those fed on plants without fungal inoculation. Plant growth was promoted when seeds were inoculated with fungi, and the fungicide did not affect any evaluated parameter. Coffee seed inoculation with and appears to provide protection against CLMs and promote growth improvement.
（肉座菌目：麦角菌科）具有多功能的生命周期，可作为植物内生菌定殖，并作为昆虫病原真菌发挥作用。 并且 可与咖啡植株建立关联，并增强对咖啡的主要害虫——咖啡潜叶蛾（CLM）的防护能力（ ）。这种关联将是一种易于实施的生物防治选择。在此，我们测试了用从巴西塞拉多咖啡作物土壤中采集的 和 接种咖啡种子以防治CLM并使用一种常用杀菌剂促进植物生长的潜力。我们在温室中进行了实验，待幼苗生长后，将它们放置在一个装有两对CLMs的笼子里。我们评估了CLM的发育时间、繁殖情况以及植物生长性状。我们观察到，以接种了两种分离株的植物为食时，CLMs的发育时间更长。此外，与取食未接种真菌植物的CLMs相比，其产卵量更少。用真菌接种种子可促进植物生长，并且该杀菌剂未影响任何评估参数。用 和 接种咖啡种子似乎可为防治CLMs提供保护并促进生长改善。
