Beris Evangelos, Venios Xenophon, Papachristos Dimitrios, Ponchon Mathilde, Kontodimas Dimitrios, Korkas Elias, Banilas Georgios, Reineke Annette
Department of Wine, Vine and Beverage Sciences, School of Food Sciences, University of West Attica, 12243 Egaleo, Athens, Greece.
Laboratory of Agricultural Entomology, Department of Entomology and Agricultural Zoology, Benaki Phytopathological Institute, 14561 Kifissia, Athens, Greece.
Microorganisms. 2025 Jul 10;13(7):1630. doi: 10.3390/microorganisms13071630.
Entomopathogenic fungi (EPF) are substantial biocontrol agents reducing the populations of economically important pests in numerous crops. Recent findings indicate that their role in agroecosystems is more complex and extends to affecting plant physiology and growth. This study examined the effects of and , as well as Salicylic acid (SA), on physiological parameters of grapevine ( cv. Sauvignon Blanc). Additionally, the impact of SA on spore germination and pathogenicity of EPF against larvae of the European grapevine moth () was tested. Foliar application of EPF was found to increase the electron transport rate (ETR) from PSII to PSI, indicating higher photosynthetic activity compared to control plants. EPF also elevated the transpiration rate (E) and stomatal conductance (gs). In contrast, SA treatments decreased E and gs, while the high dose (10 mM) exhibited reduced Fv/Fm value, accompanied by phytotoxic spots on leaves. Spore germination of both fungi was significantly reduced only by the SA concentration of 2 mM, while 0.5 and 1 mM did not affect germination. Combination EPF and SA treatments presented the highest larval mortality of (87.5% at 28 °C and 77.5% at 24 °C for and , respectively). However, SA reduced larval mycosis in most cases. Overall, the results suggest that EPF and SA can be co-applied and included in vineyard integrated strategies to support grapevine health.
昆虫病原真菌（EPF）是重要的生物防治剂，可减少多种作物上具有经济重要性的害虫数量。最近的研究结果表明，它们在农业生态系统中的作用更为复杂，还延伸至影响植物生理和生长。本研究考察了[两种昆虫病原真菌名称未给出]以及水杨酸（SA）对葡萄（品种为长相思）生理参数的影响。此外，还测试了SA对EPF孢子萌发以及对欧洲葡萄蠹蛾幼虫致病性的影响。结果发现，叶面喷施EPF可提高从光系统II到光系统I的电子传递速率（ETR），表明与对照植株相比具有更高的光合活性。EPF还提高了蒸腾速率（E）和气孔导度（gs）。相比之下，SA处理降低了E和gs，而高剂量（10 mM）处理的Fv/Fm值降低，叶片出现了植物毒性斑点。仅2 mM的SA浓度显著降低了两种真菌的孢子萌发，而0.5 mM和1 mM的SA浓度对萌发没有影响。EPF与SA联合处理对欧洲葡萄蠹蛾幼虫的致死率最高（[两种昆虫病原真菌名称未给出]在28℃时为87.5%，在24℃时分别为77.5%）。然而，在大多数情况下，SA降低了幼虫的真菌病感染率。总体而言，结果表明EPF和SA可以共同施用，并纳入葡萄园综合防治策略以维护葡萄健康。
