Smedile Daniele, Iurescia Manuela, Carfora Virginia, Cocumelli Cristiano, Palmerini Tiziana, Diaconu Elena Lavinia, Congiu Ilaria, Donati Valentina, Stravino Fiorentino, Sorbara Luigi, Romano Erica, Caprioli Andrea, Battisti Antonio
General Diagnostic Department, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "M. Aleandri", 00178 Rome, Italy.
Veterinary Practitioner, 00165 Rome, Italy.
Pathogens. 2024 Sep 11;13(9):785. doi: 10.3390/pathogens13090785.
(homotypic synonym: ) represents an ungrouped thermotolerant rapidly growing mycobacteria (RGM) species occasionally associated with infections and disease in humans. In this report, we describe a case of pyogranulomatous dermatitis and panniculitis due to in an immunocompetent adult cat. To the best of our knowledge, this represents the first report of infection in animals. We also report the results of the in-depth genome characterization of the isolate using a combined short- and long-read whole-genome sequencing (WGS) approach. We observed the lack of acquired-resistance genes and no evidence of mutations in housekeeping genes associated with resistance to rifampicin and isoniazid. We detected some virulence factors in our isolate, such as some associated with the interaction of mycobacteria with host cells, and the presence of multiple copies of heavy metal resistance genes (B, R, and L/L). In conclusion, should be included among the RGM species associated with feline subcutaneous atypical mycobacteriosis (SAM). A reliable and fast RGM laboratory identification and characterization is important not only for an accurate etiological diagnosis but also for a correct approach to SAM treatment options.
（同物异名：）代表一种未分类的耐热快速生长分枝杆菌（RGM）物种，偶尔与人类感染和疾病相关。在本报告中，我们描述了一例免疫功能正常的成年猫因感染导致的脓性肉芽肿性皮炎和脂膜炎。据我们所知，这是动物感染的首例报告。我们还报告了使用短读长和长读长全基因组测序（WGS）相结合的方法对分离株进行深入基因组特征分析的结果。我们观察到缺乏获得性耐药基因，且与利福平和异烟肼耐药相关的管家基因没有突变证据。我们在分离株中检测到一些毒力因子，例如一些与分枝杆菌与宿主细胞相互作用相关的因子，以及重金属抗性基因（B、R和L/L）的多个拷贝。总之，应将其纳入与猫皮下非典型分枝杆菌病（SAM）相关的RGM物种中。可靠且快速的RGM实验室鉴定和特征分析不仅对于准确的病因诊断很重要，而且对于正确选择SAM治疗方案也很重要。
