Ostróżka-Cieślik Aneta, Strasser Claire, Dolińska Barbara
Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Jedności Street 10, 41-200 Sosnowiec, Poland.
NETZSCH-Geratebau GmbH, Wittelsbacherstraße 42, 95100 Selb, Germany.
Polymers (Basel). 2024 Sep 16;16(18):2619. doi: 10.3390/polym16182619.
This work is part of the current research trend to develop a hydrogel carrier of insulin to promote wound healing. Topically applied insulin promotes keratinocyte proliferation and migration, increases collagen synthesis, reduces inflammation and oxidative stress, and exhibits antimicrobial activity. The aim of this study was to design an insulin hydrogel matrix based on selected cellulose derivatives (methylcellulose, hydroxyethylcellulose, and hydroxypropylmethylcellulose) and chitosan. Rheological parameters of the formulations were evaluated using rotational rheometry and an oscillation test. Textural tests were performed. In vitro pharmaceutical insulin availability studies were carried out using the innovative Strat-M membrane to imitate the skin barrier. It was found that the pharmaceutical formulation of insulin based on chitosan and methylcellulose showed an acceptable balance between rheological and textural parameters and ease of application. The API was released from the carrier in a prolonged manner, eliminating the need to apply the formulation several times per day. The developed hydrogel shows potential for use in clinical practice.
这项工作是当前研究趋势的一部分，即开发一种胰岛素水凝胶载体以促进伤口愈合。局部应用胰岛素可促进角质形成细胞的增殖和迁移，增加胶原蛋白的合成，减轻炎症和氧化应激，并具有抗菌活性。本研究的目的是基于选定的纤维素衍生物（甲基纤维素、羟乙基纤维素和羟丙基甲基纤维素）和壳聚糖设计一种胰岛素水凝胶基质。使用旋转流变仪和振荡试验评估制剂的流变学参数。进行了质地测试。使用创新的Strat-M膜模拟皮肤屏障进行了体外药物胰岛素可用性研究。结果发现，基于壳聚糖和甲基纤维素的胰岛素药物制剂在流变学和质地参数以及易于应用之间表现出可接受的平衡。活性药物成分从载体中持续释放，无需每天多次应用该制剂。所开发的水凝胶在临床实践中显示出应用潜力。
