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来自捷克的糖芥潜隐病毒分离株的基因组序列 。 (原英文文本不完整,翻译可能不太准确,完整准确的翻译需补充完整原文内容)

Genomic Sequence of a Czech Isolate of Erysimum Latent Virus from .

作者信息

Ben Mansour Karima, Špak Josef, Komínek Petr, Zouhar Miloslav, Ryšánek Pavel, Gibbs Adrian J

机构信息

Ecology, Diagnostics and Genetic Resources of Agriculturally Important Viruses, Fungi and Phytoplasmas, Crop Research Institute, Drnovská 507, 161 06 Prague, Czech Republic.

Department of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech Republic.

出版信息

Plants (Basel). 2024 Sep 11;13(18):2554. doi: 10.3390/plants13182554.

Abstract

The Erysimum latent virus (ELV), a tymovirus, was first isolated from several wild and cultivated brassicas in Germany. Its virions were shown to be serologically distinct from those of the turnip yellow mosaic virus (TYMV), which is also found in wild and cultivated plants in several European countries but also in other parts of the world. TYMV and ELV were among the first plant viruses to have had their genomes sequenced, and when other tymovirus genomes were sequenced, it was found that, in phylogenies, ELV is probably the basal outlier to all other tymoviruses. Here, we report the near-complete genomic sequence of another isolate of ELV from Czechia. This isolate was found in 1990 in plants showing mosaic symptoms. It was detected using ELISA tests and electron microscopy. We have now sequenced the full coding sequence of this isolate using contemporary high throughput methods and found that the German and Czech isolates of ELV are closely related and are of the same virus species.

摘要

糖芥潜隐病毒(ELV)是一种番茄病毒,最初从德国的几种野生和栽培芸苔属植物中分离出来。研究表明,其病毒粒子在血清学上与芜菁黄花叶病毒(TYMV)不同,TYMV也存在于欧洲几个国家的野生和栽培植物中,在世界其他地区也有发现。TYMV和ELV是最早进行基因组测序的植物病毒之一,当对其他番茄病毒基因组进行测序时,发现在系统发育中,ELV可能是所有其他番茄病毒的基部异常值。在此,我们报告了来自捷克的另一株ELV分离株的近乎完整的基因组序列。该分离株于1990年在表现出花叶症状的植物中发现。它通过酶联免疫吸附测定试验和电子显微镜检测到。我们现在使用当代高通量方法对该分离株的完整编码序列进行了测序,发现ELV的德国和捷克分离株密切相关,属于同一病毒种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1a7/11434642/393e0b0405b7/plants-13-02554-g001.jpg

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