Department of Plant Virology, Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9, 84505, Bratislava, Slovakia,
Arch Virol. 2014 Aug;159(8):2103-7. doi: 10.1007/s00705-014-2031-5. Epub 2014 Mar 6.
Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumulation of 21 indel mutations in ORF1, resulting in a localized high divergence in the encoded amino acid sequences. An elevated divergence in the 5' extremity of the GPGV genomes is suggestive of a recombination between Slovak isolates and grapevine berry inner necrosis virus. RT-PCR allowed the frequent detection of closely related GPGV isolates in grapevines from Slovakia and the Czech Republic.
对来自斯洛伐克的三个灰比诺葡萄病毒（GPGV）分离株的全基因组序列分析表明，它们的异质性很低（达到 1.7%），与意大利的 NC_015782 分离株密切相关（4.2-4.5%的差异）。对斯洛伐克和意大利分离株的比较表明，ORF1 中存在不寻常的 21 个插入缺失突变，导致编码氨基酸序列的局部高度差异。GPGV 基因组 5'端的高度差异提示斯洛伐克分离株与葡萄浆果内坏死病毒之间发生了重组。RT-PCR 允许频繁检测到来自斯洛伐克和捷克共和国的葡萄藤中密切相关的 GPGV 分离株。
