Hu Yiqun, Chen Qingqing, Zhang Aifang, Zhang Liyuan, Dong Hansong
Anhui Province Key Laboratory of Pesticide Resistance Management on Grain and Vegetable Pests, Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
National Key Laboratory of Wheat Improvement, College of Plant Protection, Shandong Agricultural University, Taian 271018, China.
Plants (Basel). 2024 Sep 14;13(18):2576. doi: 10.3390/plants13182576.
Small non-coding RNAs (sRNAs) are pivotal post-transcriptional regulatory factors influencing biological activity. Studies on the rice bacterial blight pathogen pathovar strain PXO99, previously identified a virulence-associated sRNA, trans3287. A mutant strain lacking this sRNA, named SK01, resulted in markedly diminished virulence towards rice. This study aims to further elucidate the underlying bacterial virulent function of trans3287. The expression of trans3287 was quantified in virulence-inducing and standard nutritional conditions to clarify its production mechanism. The detection of virulence-associated genes revealed that trans3287 regulated the synthesis processes of extracellular polysaccharides, lipopolysaccharides, and the type III secretion system. Moreover, bioinformatics prediction and quantitative PCR indicated a potential direct target of trans3287, , encoding cardiolipin synthase. A dual-plasmid system fusing with GFP tag and protein immunoblotting confirmed that sRNA trans3287 negatively regulated . Bacterial biofilms demonstrated trans3287 regulated the disruption of biofilm integrity through cardiolipin synthase. This study provides preliminary insights into the mechanistic underpinnings of the role of sRNA trans3287 in mediating bacterial virulence through cardiolipin synthase.
小非编码RNA（sRNAs）是影响生物活性的关键转录后调控因子。对水稻白叶枯病菌致病型菌株PXO99的研究，先前已鉴定出一种与毒力相关的sRNA，即trans3287。缺失这种sRNA的突变菌株SK01对水稻的毒力显著降低。本研究旨在进一步阐明trans3287潜在的细菌毒力功能。在诱导毒力和标准营养条件下对trans3287的表达进行定量，以阐明其产生机制。对与毒力相关基因的检测表明，trans3287调控细胞外多糖、脂多糖和III型分泌系统的合成过程。此外，生物信息学预测和定量PCR表明trans3287的一个潜在直接靶标，即编码心磷脂合酶的基因。融合GFP标签的双质粒系统和蛋白质免疫印迹证实sRNA trans3287对该基因起负调控作用。细菌生物膜表明trans3287通过心磷脂合酶调控生物膜完整性的破坏。本研究为sRNA trans3287通过心磷脂合酶介导细菌毒力作用的机制提供了初步见解。
