National International Joint Research Center for Animal Immunology, School of Animal Medicine, Henan Agricultural University, Zhengzhou 450046, China.
Key Laboratory of Animal Pathogenesis and Biosafety, Ministry of Education, School of Animal Medicine, Henan Agricultural University, Zhengzhou 450046, China.
Viruses. 2024 Aug 29;16(9):1373. doi: 10.3390/v16091373.
The porcine reproductive and respiratory syndrome virus (PRRSV) belongs to the Arteriviridae family and is a single-stranded, positively stranded RNA virus. The currently available PRRSV vaccines are mainly inactivated and attenuated vaccines, yet none of the commercial vaccines can provide comprehensive, long-lasting, and effective protection against PRRSV. SR717 is a pyridazine-3-carboxamide compound, which is commonly used as a non-nucleoside STING agonist with antitumor and antiviral activities. Nevertheless, there is no evidence that SR717 has any antiviral effects against PRRSV. In this study, a dose-dependent inhibitory effect of SR717 was observed against numerous strains of PRRSV using qRT-PCR, IFA, and WB methods. Furthermore, SR717 was found to stimulate the production of anti-viral molecules and trigger the activation of the signaling cascade known as the stimulator of interferon genes (STING) pathway, which contributed to hindering the reproduction of viruses by a certain margin. Collectively, these results indicate that SR717 is capable of inhibiting PRRSV infection in vitro and may have potential as an antiviral drug against PRRSV.
猪繁殖与呼吸综合征病毒（PRRSV）属于动脉炎病毒科，是一种单链、正链 RNA 病毒。目前可用的 PRRSV 疫苗主要是灭活疫苗和减毒疫苗，但没有一种商业疫苗能对 PRRSV 提供全面、持久和有效的保护。SR717 是一种哒嗪-3-甲酰胺化合物，通常用作具有抗肿瘤和抗病毒活性的非核苷 STING 激动剂。然而，没有证据表明 SR717 对 PRRSV 有任何抗病毒作用。在这项研究中，使用 qRT-PCR、IFA 和 WB 方法观察到 SR717 对多种 PRRSV 株具有剂量依赖性抑制作用。此外，SR717 被发现能刺激抗病毒分子的产生，并触发干扰素基因刺激物（STING）途径的信号级联反应的激活，这有助于在一定程度上抑制病毒的繁殖。总之，这些结果表明，SR717 能够抑制 PRRSV 在体外的感染，可能有潜力成为抗 PRRSV 的抗病毒药物。
