Song Xin-Qi, Zhao Xin-Yi, Chen Wen-Shuang, Yang Li, Liu Dong-Yu, Chen Ya-Ping
College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, PR China.
College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, PR China.
Virology. 2025 Feb;603:110382. doi: 10.1016/j.virol.2024.110382. Epub 2025 Jan 6.
Porcine reproductive and respiratory syndrome virus (PRRSV) is a viral infectious disease that can cause infection in pigs of different ages. The condition known as porcine reproductive and respiratory syndrome poses a serious risk to the world's pig business and results in significant financial losses. Fuzhengjiedu San (FZJDS) is a traditional Chinese medicine compound, the main components include:Radix Isatidis, Radix Astragali and Herba Epimedii. It has been widely used in clinical and experimental studies, showing a wide range of biological activity. However, it is not clear whether FZJDS has anti-PRRSV activity. We observed that FZJDS had significant antiviral activity in Marc-145 cells. And FZJDS could inhibit viral infection in the stages of viral internalization and replication. Furthermore, FZJDS can inhibit PRRSV replication by inhibiting the p53 signaling pathway to affect autophagy, and FZJDS can also inhibit PRRSV replication by inhibiting the PI3K/Akt pathway.We showed in this work that FZJDS inhibits PRRSV replication in vitro and offers a novel therapeutic approach for PRRSV infection.
猪繁殖与呼吸综合征病毒（PRRSV）是一种能感染不同年龄段猪的病毒性传染病。猪繁殖与呼吸综合征给全球养猪业带来严重风险，并造成重大经济损失。扶正解毒散（FZJDS）是一种中药复方，主要成分包括：板蓝根、黄芪和淫羊藿。它已广泛应用于临床和实验研究，显示出广泛的生物活性。然而，扶正解毒散是否具有抗PRRSV活性尚不清楚。我们观察到扶正解毒散在Marc-145细胞中具有显著的抗病毒活性。并且扶正解毒散能在病毒内化和复制阶段抑制病毒感染。此外，扶正解毒散可通过抑制p53信号通路影响自噬来抑制PRRSV复制，扶正解毒散还可通过抑制PI3K/Akt通路抑制PRRSV复制。我们在这项研究中表明，扶正解毒散在体外抑制PRRSV复制，为PRRSV感染提供了一种新的治疗方法。
