Li Lan, Tian Xiaoning, Chen Jin, Li Pengcheng, Zheng Qisheng, Hou Jibo
National Research, Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, People's Republic of China.
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China.
Arch Virol. 2018 Dec;163(12):3317-3325. doi: 10.1007/s00705-018-4029-x. Epub 2018 Sep 15.
Porcine reproductive and respiratory syndrome virus (PRRSV) is a pathogen that severely disrupts swine production. Despite sustained efforts, the disease is still endemic, with high mortality and morbidity. New antiviral strategies to control PRRSV are needed. Griffithsin, a red algal lectin, has potent antiviral effect on several human enveloped viruses, but this effect has not been demonstrated on PRRSV. Here, we first tested the in vitro antiviral activity of Griffithsin against PRRSV. Griffithsin exerted strong saccharide-dependent antiviral activity against PRRSV, probably through interactions with glycans on the surface of PRRSV that interfered with virus entry. Furthermore we revealed that Griffithsin's action on PRRSV involved blocking viral adsorption, and it had no effect on viral penetration. Besides Our findings also suggested that Griffithsin may interfere with cell-to-cell spread to prevent virus transmission. The remarkable potency profile of Griffithsin supports its potential value as an antiviral agent against PRRSV.
猪繁殖与呼吸综合征病毒（PRRSV）是一种严重扰乱养猪生产的病原体。尽管人们持续努力，但该疾病仍然流行，死亡率和发病率很高。需要新的抗病毒策略来控制PRRSV。红藻凝集素格里菲斯菌素对几种人类包膜病毒具有强大的抗病毒作用，但尚未在PRRSV上得到证实。在这里，我们首先测试了格里菲斯菌素对PRRSV的体外抗病毒活性。格里菲斯菌素对PRRSV具有强大的依赖糖类的抗病毒活性，可能是通过与PRRSV表面的聚糖相互作用来干扰病毒进入。此外，我们发现格里菲斯菌素对PRRSV的作用涉及阻断病毒吸附，并且对病毒穿透没有影响。此外，我们的研究结果还表明，格里菲斯菌素可能会干扰细胞间传播以防止病毒传播。格里菲斯菌素显著的效力特征支持了其作为抗PRRSV抗病毒剂的潜在价值。
