National Institute for Public Health and the Environment, Centre for Infectious Disease Control, 3721MA Bilthoven, The Netherlands.
Department of Pathology, Amsterdam UMC Location Vrije Universiteit Amsterdam, 1007MB Amsterdam, The Netherlands.
Viruses. 2024 Aug 29;16(9):1381. doi: 10.3390/v16091381.
HPV16 is occasionally detected in vaccinated women who received the bivalent HPV16/18 vaccine, usually at low viral loads. This study explored potential differences in HPV16 variants between vaccinated and unvaccinated women. HPV16-postive viral loads were detected in 1.9% (17/875) and 13% (162/760) of vaccinated and unvaccinated women, respectively, showcasing the vaccine's high efficacy. The L1, E6, and URR regions of HPV16 were sequenced from genital swabs from 16 vaccinated and 25 unvaccinated women in the HAVANA (HPV Among Vaccinated And Non-vaccinated Adolescents) study. The majority of HPV16 variants from vaccinated and unvaccinated women clustered similarly with sub-lineages A1 and A2. Additionally, a separate cluster within lineage A was found, with the variants sharing the L1-located SNP A753G (synonymous) and the URR-located SNP T340C, which did not occur in the other variants. Furthermore, four variants from vaccinated women had relatively long branches, but were not characterized by specific SNPs. The frequency of G712A in the URR was the only SNP observed to be marginally higher among vaccinated women than unvaccinated women. Non-synonymous SNPs T266A in the FG-loop of L1 and L83V in E6 were common among variants from vaccinated and unvaccinated women, but present in similar frequencies. In conclusion, the detection of HPV16 in vaccinated (and unvaccinated) women seemed to be the result of random circulation within this study population.
HPV16 偶尔会在接种了二价 HPV16/18 疫苗的女性中被检测到，通常病毒载量较低。本研究探讨了接种疫苗和未接种疫苗女性之间 HPV16 变体的潜在差异。在接种组和未接种组中，HPV16 阳性病毒载量分别为 1.9%（17/875）和 13%（162/760），显示出疫苗的高功效。在 HAVANA（HPV 在接种和未接种青少年中的研究）研究中，从 16 名接种疫苗和 25 名未接种疫苗的女性的生殖器拭子中对 HPV16 的 L1、E6 和 URR 区进行了测序。接种疫苗和未接种疫苗的女性的 HPV16 变体主要以亚谱系 A1 和 A2 聚类相似。此外，还在谱系 A 内发现了一个单独的聚类，变体共享位于 L1 的 SNP A753G（同义）和位于 URR 的 SNP T340C，而其他变体则没有。此外，来自接种疫苗的女性的四个变体具有相对较长的分支，但没有被特定的 SNP 所描述。在 URR 中观察到的 G712A 频率是唯一一个在接种组中比未接种组略高的 SNP。L1 的 FG-环中的非同义 SNP T266A 和 E6 中的 L83V 在接种疫苗和未接种疫苗的女性中变体中很常见，但出现的频率相似。总之，在接种疫苗的（和未接种疫苗的）女性中检测到 HPV16 似乎是该研究人群中随机循环的结果。
