Department of Plant Pathology, University of California-Davis, Davis, CA 95616, USA.
Foundation Plant Services, University of California-Davis, Davis, CA 95616, USA.
Viruses. 2024 Sep 13;16(9):1457. doi: 10.3390/v16091457.
The grapevine fleck virus (GFkV) is a ubiquitous grapevine-infecting virus found worldwide, is associated with the grapevine fleck complex, and is often found in mixed infections with viruses of the grapevine leafroll complex and/or vitiviruses. Although GFkV has been studied for a long time, limited sequence information is available in the public databases. In this study, the GFkV sequence data available in GenBank and data generated at the Foundation Plant Services, University of California, Davis, were used to perform nucleotide sequence comparisons, construct a phylogenetic tree, and develop a new RT-qPCR assay. Sequence comparisons showed high genetic diversity among the GFkV isolates, and the phylogenetic analyses revealed a new group comprised of GFkV isolates identified in the present study. A new assay, referred to as GFkV-CP, was designed and validated using an existing GFkV positive control together with 11 samples known to be infected with combinations of different marafiviruses and maculaviruses but not GFkV. In addition, the newly designed assay was used in a field survey to screen grapevines from diverse geographical locations that are maintained at the United States Department of Agriculture (USDA) National Clonal Germplasm Repository (NCGR) in Winters, CA.
葡萄扇叶病毒（GFkV）是一种普遍存在于世界各地的感染葡萄的病毒，与葡萄扇叶复合病相关，通常与葡萄卷叶复合病病毒和/或葡萄病毒混合感染。尽管 GFkV 已经研究了很长时间，但公共数据库中可用的序列信息有限。在本研究中，使用 GenBank 中提供的 GFkV 序列数据和加利福尼亚大学戴维斯分校基金会植物服务处生成的数据，进行核苷酸序列比较、构建系统发育树，并开发新的 RT-qPCR 检测方法。序列比较表明，GFkV 分离株之间具有高度遗传多样性，系统发育分析显示，本研究中鉴定的 GFkV 分离株构成了一个新的群组。设计并验证了一种新的检测方法，称为 GFkV-CP，该方法使用现有的 GFkV 阳性对照以及 11 个已知感染不同甲病毒和马病毒组合但未感染 GFkV 的样本进行验证。此外，该新设计的检测方法还用于对加利福尼亚州温特斯美国农业部（USDA）国家无性系种质资源库（NCGR）中来自不同地理位置的葡萄进行田间调查。
