School of Food Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225127, China.
College of Agricultural and Environmental Sciences, University of California, California 95616, United States.
Food Chem. 2025 Jan 15;463(Pt 3):141436. doi: 10.1016/j.foodchem.2024.141436. Epub 2024 Sep 25.
Deoxynivalenol (DON) contamination in food products significantly threatens human health, necessitating a reliable and sensitive detection method. This study aims to develop a simple, low-cost, and effective electrochemical immunoassay method for detecting DON based on the nickel‑iron bimetallic Prussian blue analog (NiFe PBA). The NiFe PBA nanozymes with high peroxidase-like activity were synthesized using an environmentally friendly chemical precipitation method. In the presence of hydrogen peroxide (HO), the current change of thionine oxidation initiated by NiFe PBA nanozymes can be exploited to diagnose DON. Under optimal conditions, the proposed method achieved quantitative detection of DON in the range of 10-10 pg mL with a detection limit of 4.5 pg mL (S/N = 3), demonstrating excellent selectivity, reproducibility, and stability. In addition, the DON immunosensor provides satisfactory results for the detection in real samples, demonstrating the feasibility of the proposed sensor in detecting of DON in such products.
脱氧雪腐镰刀菌烯醇（DON）污染食品产品严重威胁人类健康，因此需要一种可靠且灵敏的检测方法。本研究旨在开发一种简单、低成本、有效的电化学免疫分析方法，用于基于镍铁双金属普鲁士蓝类似物（NiFe PBA）检测 DON。使用环保的化学沉淀法合成了具有高过氧化物酶样活性的 NiFe PBA 纳米酶。在存在过氧化氢（HO）的情况下，可以利用 NiFe PBA 纳米酶引发的硫堇氧化的电流变化来诊断 DON。在最佳条件下，该方法实现了 DON 在 10-10 pg mL 范围内的定量检测，检测限为 4.5 pg mL（S/N = 3），表现出优异的选择性、重现性和稳定性。此外，DON 免疫传感器在实际样品检测中提供了令人满意的结果，表明该传感器在检测此类产品中的 DON 具有可行性。
